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EC number: -
CAS number: -
Two experiments were performed. Because of the algae celts in the
samples after 72 hours, sample preparation via solid phase extraction
wasn't possible (cartridges became clogged during extraction). Therefore
the first experiment was aborted. In the second experiment, the samples
after 72 hours were centrifuged. The results of the first study aren't
reported- The main study was performed as a limit test using one
concentration in the range of water solubility. The water solubility was
determined as approximately 7 µg/L. At the start and at the end of the
test, the content of the test item in the test solutions was determined
using GC. In both experiments the measured values at the start of the
test were in the range of water solubility. As the test item wasn't
stable in under test conditions, the measured concentrations test item
after 72 hours were lower than LOQ. The treatment was used to incubate
the unicellular freshwater green algae Desmodesmus subspicatus for a
period of 72 hours. The cell concentration of each replicate was
determined by measuring the absorption of the cuvettes at 440 nm every
24 hours with a spectral photometer. With these measured values, the
number of cells was calculated (linear correlation between cell
concentration and absorption given). Then the growth rate, the area
under the growth cunte (AUC) and the Yield were determined. The
determination of the results was based on the limit of water solubility.
The EC50s of potassium dichromate was tested in a current reference
test. The values were within the normal range of the laboratory.
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