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Administrative data

Key value for chemical safety assessment

Additional information

Benzoguanamine was tested using the Ames test according to OECD TG 471 and GLP and showed no mutagenic activity neither with nor without exogenous metabolic activation up to 5000 µg/plate. In three studies no evidence of mutagenic activity of Benzoguanamine with and without mutagenic activation was observed (Anonym 1988, Clare 1988, Nakajima 1999).

In addition, a study was preformed to investigate the potential of Benzoguanamine to induced gene mutation at the mouse lymphoma cells line L 5178 TK+/- according to OECD TG 476 and GLP in concentration up to 2500 µg/mL in the presence and absence of S9 -mix. Cytotoxicity was observed in the test with metabolic activation above the solubility limit. No Cytotoxicity was found in the test with metabolic activation within the solubility limit and in the test without metabolic activation (Jenkinson 1993).

According to OECD TG 473 and GLP two test were preformed.

In the first test Benzoguanamine was tested for chromosomal abberation with human lymphocytes cells in concentrations up to 2500 µg/mL with and without metabolic activation system. There was no evidence for cytotoxicity with metabolic activation system within and above the solubility limit. Cytotoxicity was detected without metabolic activation system within and above the solubility limit (Pateman 1993).

In the second test Benzoguanamine was tested for chromosomal abberation in Chinese Hamster Lung (CHL) cells with and without metabolic activation system. Cytotoxicity was observed without metabolic activation system. Findings for cytotoxicity with metabolic activation system were ambiguous (Nakajima 1999).

The cytogenetic effect observed in in vitro assays however, could not be reproduced in the micronucleus tests in vivo (Honarvar 2000).


Short description of key information:
Benzoguanamine revealed no mutagenic activity in the Ames test according to OECD TG 471 and GLP.
Cytogenic effects were observed when tested according to OECD TG 476 and 473. The cytogenetic effects, observed in in-vitro assays, could not be reproduced in the in vivo micronucleus tests. Based on these results, it could be concluded that Benzoguanamine is not genotoxic.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

For genotoxicity Benzoguanamine is not classified in accordance to Regulation (EC) No 1272/2008, respectively Regulation (EC) No 1272/2008.