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EC number: 475-900-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24-26 Apr. 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted according to an internationally recognised method, and under GLP. The substance is adequately characterised with its purity. Therefore full validation applies.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD, 2004
- Deviations:
- yes
- Remarks:
- Climate chamber temperature between 19.4 and 20.8°C (thus varying more than +/- 1°C). No influence on the results because 20.8°C recorded in only one occasion (air temperature recorded once every hour).
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- As ETFBO hydrolyses quickly (T1/2 is < 5 min at the test temperature), the concentration in the test solutions was based on the hydrolysis product, 4,4,4-trifluoro-3-oxobutanal (Eicher, 2006).
- Details on sampling:
- - Concentrations: Concentrations were analysed in both treatments: control (Elendt M4 medium) and exposed to 100 mg/L.
- Sampling method: Samples of 100 μL were taken in triplicate:
* from the diluted stock solution and Elendt M4 medium at the beginning of the study,
* from experimental beakers at the end of the study (on day 2).
- Sample storage conditions before analysis: The samples were analysed on the day of sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: On the day of test initiation, a stock solution was prepared by dissolving 260 mg ETFBO in 250 mL Elendt M4 medium resulting in an ETFBO concentration of 1.04 g/L. This stock solution was diluted ten times with Elendt M4 medium resulting in a stock solution of 104 mg/L.
- Controls: Elendt M4 medium.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No undissolved test substance was visible.
- Other relevant information: The test solution with ETFBO was slightly yellow. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea.
- Age at study initiation: 0 - 24h at the start of the test.
- Method of breeding: The water fleas were in culture since 4 February 2003. Water fleas of the same age were cultured in vessels with 2 L Elendt M4 medium. They were fed with an algal suspension of Chlorella vulgaris. The water fleas were cultured in a climate chamber with a temperature setpoint of 20°C. The light regime was 16h light and 8h darkness per day. The light was switched on at 6 a.m. Each week, a new culture of water fleas was started and water fleas older than 2 weeks were removed.
- Source: Water fleas used in this test originate from a culture received on 4 February 2003 from Aquasense b.v., Amsterdam, The Netherlands.
- Age of parental stock: not older than 2 weeks.
No further data. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- No data
- Test temperature:
- Control:
Day 0 (test initiation): 19.7°C
Day 2 (test end): 20.3°C
100 mg/L:
Day 0 (test initiation): 20.2°C
Day 2 (test end): 20.4°C - pH:
- Control:
Day 0 (test initiation): 8.1
Day 2 (test end): 8.2
100 mg/L:
Day 0 (test initiation): 6.5
Day 2 (test end): 7.5 - Dissolved oxygen:
- Control:
Day 0 (test initiation): 8.9 mg/L
Day 2 (test end): 8.8 mg/L
100 mg/L:
Day 0 (test initiation): 9.0 mg/L
Day 2 (test end): 8.8 mg/L - Nominal and measured concentrations:
- Nominal concentrations: 0, and 100 mg/L.
Measured concentrations: 0.04 mg/L to not detected, and 93.9 mg/L (mean of concentrations measured on day 0 and day 2).
N.B.1: As ETFBO hydrolyses quickly (T1/2 is < 5 min at the test temperature), the concentration in the test solutions was based on the hydrolysis product, 4,4,4-trifluoro-3-oxobutanal (Eicher, 2006).
N.B.2: On day 0, a small amount (< lowest calibration standard) of 0.04 mg/L was measured in the control. As the substance was also determined in the blank sample (mobile phase), and no substance was determined in the samples on day 2, it was concluded that the mobile phase was contaminated during sampling on day 0.
N.B.3: The difference between the nominal and the mean measured concentrations was < 20% and for this reason the effect concentrations were based on nominal concentration. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers.
- Volume of solution: 200 mL.
- No. of organisms per vessel: 10.
- No. of vessels per concentration (replicates): 6.
- No. of vessels per control (replicates): 6.
No further data.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium prepared in purified water (purified water was prepared according to the methods of European Pharmacopeia and the USP Purified Water).
- Culture medium different from test medium: No.
- Intervals of water quality measurement: At test initiation, the pH, temperature and oyxgen content were measured in the Elendt medium and the stock solution. At test termination, the pH, temperature and oxygen content was measured in one glass beaker for both treatments.
No further data.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: The light regime was 16h light and 8h dark (light on from 6 a.m. to 10 p.m.).
No further data.
EFFECT PARAMETERS MEASURED
The number of mobile and immobile water fleas and the number of water fleas showing sublethal effects were recorded 24 and 48 hours after test initiation. Immobile water fleas are those animals which are not able to swim within 15 seconds after gentle agitation of the glass beaker.
RANGE-FINDING STUDY
- Spacing factor for test concentrations: Not applicable (limit test).
- Range finding study : yes, not detailed except that results allowed to select a limit test design (therefore, it is deduced that no adverse effect was recorded during the range-finding study). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: The difference between the nominal and the mean measured concentration was < 20% and for this reason the endpoint was based on nominal concentration.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: The difference between the nominal and the mean measured concentration was < 20% and for this reason the endpoint was based on nominal concentration.
- Details on results:
- BIOLOGICAL RESULTS
No immobility or sublethal effects were observed in the control glass beakers and in the glass beakers containing 100 mg/L ETFBO.
ANALYTICAL RESULTS
As ETFBO hydrolyses quickly (T1/2 is < 5 min at the test temperature), the concentration in the test solutions was based on the hydrolysis product, 4,4,4-trifluoro-3-oxobutanal (Eicher, 2006):
Measured concentrations in the control group:
Day 0 (test initiation): a small amount (< lowest calibration standard) of 0.04 mg/L was measured in the control on day 0. As the substance was also determined in the blank sample (mobile phase), and no substance was determined in the samples on day 2, it was concluded that the mobile phase was contaminated during sampling on day 0. Because no adverse effect was observed, this was judged without effect on the study conclusions.
Day 2 (test end): not detected.
Measured concentrations in the group exposed to the nominal concentration of 100 mg/L:
Day 0 (test initiation): 99.3 mg/L.
Day 2 (test end): 88.5 mg/L.
Mean measured concentration: 93.9 mg/L.
The difference between the nominal and the mean measured concentrations was < 20% and for this reason the effect concentrations were based on nominal concentration. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes (24h-EC50 within the acceptance range of 0.6-1.7 mg/L for potassium dichromate)
.
- 24h-EC50: 1.28 mg/L (test conducted from 21 to 22 March 2006). - Reported statistics and error estimates:
- The NOEC for immobility was determined with Fisher's Exact Test (Sokal and Rohlf, 1981).
- Validity criteria fulfilled:
- yes
- Remarks:
- No immobility in the controls and dissolved oxygen concentrations at the end of the test > 3 mg/L.
- Conclusions:
- No adverse effects on water fleas were observed upon acute exposure and the 48h-EC50 (Daphnia magna) was set superior to the highest tested concentration of 100 mg/L.
- Executive summary:
The acute toxicity to daphnids was investigated in a GPL-compliant study performed according to OECD test guideline 202.
Groups of ten water fleas (Daphnia magna) were exposed for 48 hours under static conditions to a control treatment and to a treatment exposed to 100 mg/L ETFBO (nominal concentration). Mobility and sublethal effects were observed after 24 and 48 hours.
To study exposure to ETFBO, samples of test solution were taken and analysed with HPLC at the beginning (on day 0) and at the end of the test (on day 2). As ETFBO hydrolyses quickly (T1/2 is < 5 min at the test temperature), the concentration in the test solutions was based on the hydrolysis product, 4,4,4-trifluoro-3-oxobutanal (Eicher, 2006). The mean of concentrations measured on day 0 and day 2 was equal to 93.9 mg/L; meaning that the difference between nominal and measured concentrations was < 20 % and that effect concentrations could be thus based on nominal concentrations.
Because neither immobility, nor sublethal effects were observed in the group exposed to the concentration of 100 mg/L, the 48h-NOEC and 48h-EC50 were respectively set >= and > to this concentration. It can be thus concluded that initial exposure to ETFBO and then to its hydrolysis product did not cause any adverse acute effect on Daphnia magna under the present experimental conditions.
Reference
Description of key information
Because neither immobility, nor sublethal effects were observed in the group exposed to the concentration of 100 mg/L, the 48h-NOEC and 48h-EC50 were respectively set >= and > to this concentration. It can be thus concluded that initial exposure to ETFBO and then to its hydrolysis product did not cause any adverse acute effect on Daphnia magna under the present experimental conditions.
Key value for chemical safety assessment
Additional information
An experimental study, conducted according to OECD guideline and GLP, is available. It is considered as fully reliable, and the result is retained as key data.
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