γ-valerolactone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GPL guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2021

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

γ-valerolactone

EC Number:

203-569-5

EC Name:

γ-valerolactone

Cas Number:

108-29-2

Molecular formula:

C5H8O2

IUPAC Name:

5-methyloxolan-2-one

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock test solution (120 mg/L) was prepared by directly adding test substance to 1L of test
medium and stirring for approximately 10 min at ambient temperature until visibly dissolved.
The solution was checked visually to ensure that the test substance was completely dissolved.
The stock solution appeared colourless-clear.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820
used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Nominal and measured concentrations:

Since the measured concentrations of the test substance in the test
solutions were within ±20% of the nominal concentrations (97.9 - 99.9 %), the
effect concentration can be expressed relative to the nominal
concentration.
This

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Material, size, headspace, fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: 184-772 lux at a wavelength of 400-750 nm;
16 hours light : 8 hours darkness

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No mortality was oberved in control and at 120 mg/L and in all replicates.

Any other information on results incl. tables

Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes