N,N'-bis(3-aminopropyl)propane-1,3-diamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data are given

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Method: BASF-test
According to OECD guideline 402, a limit test at requires one dose level of at least 2000 mg/kg bodyweight in
a group of 5 male and 5 female animals.
The test described here used one dose level of 200 mg/kg.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trimethylenediamine

EC Number:

203-702-7

EC Name:

Trimethylenediamine

Cas Number:

109-76-2

Molecular formula:

C3H10N2

IUPAC Name:

propane-1,3-diamine

Test animals

Species:

rabbit

Strain:

Vienna White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Zuchtbetrieb: M.GAUKLER, 6050 Offenbach
- Weight at study initiation: 2.9 kg (in average)

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The fur was removed from the dorsal and lateral trunk by electrical hair clipper about 15 to 24 h before application.
Only animals with healthy and intact skin were exposed (treated area: 50 cm2).
The dose is retained beneath for 24h an aluminium foil fixed by adhesive tapes.
Thereafter, excess fluid was removed.

Duration of exposure:

24 h

Doses:

200 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy was conducted on decedents and sacrificed survivors.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 200 mg/kg bw

Based on:

test mat.

Mortality:

1h: males: 0/5; females: 0/5
24h: males: 0/5; females: 0/5
48h: males: 0/5; females: 1/5
7d: males: 1/5; females: 1/5
14d: males: 2/5; females: 1/5

Clinical signs:

Apathy
Accelerated breathing and dyspnea
Blood in urine and faeces

Local irritations: Necrosis in all animals 24h after application.

Gross pathology:

Victims: Heart: Acute dilatation, right; acute stasis hyperemia. Bladder: Bloody content (2); Kidney: Renal pelvis enlarged (1), coloured like sand (1); Liver: Discolorations
Survivors: No substance related findings.

Any other information on results incl. tables

Based on the available data, the LD50 was defined according to a DOT-guidance; an exact value was not provided.