Registration Dossier
Registration Dossier
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EC number: 225-007-8 | CAS number: 4605-14-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible. Key value for chemical safety assessment
- EC50 for microorganisms:
- 720 mg/L
- EC10 or NOEC for microorganisms:
- 34 mg/L
Additional information
No experimetnal data are available for TPTA (CAS 4605 -14 -5). Therefore a read-across approach was performed to the structurally similar substance N,N’-bis(3-aminopropyl)ethylenediamine (CAS 10563 -26 -5).
The microbial toxicity of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5) was studied in two tests: 1) GLP guideline study, respiration inhibition test with activated sludge, OECD 209; 2) non-GLP guideline study, Pseudomonas putida growth inhibition according to Bringmann-Kühn, German Industrial Standards DIN 38412, part 8.
The respiration inhibition test used activated sludge as inoculum taken from a wwtp treating predominantly domestic sewage. The 3 -h EC10 was determined to be 34 mg/L, the 3 -h EC50 was 720 mg/L (BASF SE, 2012; report no. 08G0402/04G008).
In the growth inhibition test, the test species was Pseudomonas putida. The test concentrations were not analytically verified. The 17 -h EC10 was determined to be 3.97 mg/L, the 17 -h EC50 was 8.8 mg/L (BASF AG, 1990; report no. 1/89/0502). This result will be used as supporting study.
The OECD 209 study was selected as key study since this result was determined under GLP according to an international guideline. The PNECs derived from the EC10 values of both studies are very similar (OECD 209: 3.4 mg/L; P.putida: 4.0 mg/L).
It can be concluded that Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.
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