Registration Dossier
Registration Dossier
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EC number: 218-301-2 | CAS number: 2110-78-3
Endpoint summary
Administrative data
Description of key information
Daphnia sp. Acute Immobilization Test (OECD TG 202): 24- and 48-hour EC50 >100 mg/L
Alga Growth Inhibition Test (OECD TG 201): Cell yield 72-hour EyC50 54.8 mg/L, growth rate 72-hour ErC50 >100 mg/L, growth rate 72-hour ErC20 95.6 mg/L
Additional information
Toxicity to aquatic invertebrates
A Daphnia sp. Acute Immobilization Test in accordance with OECD Testing Guideline 202 and GLP principles was designed to assess the potential effects of methyl 2-hydroxyisobutyrate to the freshwater cladoceran, Daphnia magna. The study was conducted as a limit test with four replicate vessels of five daphnids per test level exposed to nominal test concentrations of 0 (water control) and 100 mg methyl 2-hydroxyisobutyrate/L, over a 48-hour static exposure period. Observations were made at 24 and 48 hours (±1 hour from test initiation) for daphnid immobility (inability to swim within approximately 15 seconds after gentle agitation of the test vessel) and any changes in behavior or appearance. Temperatures during the exposure period ranged from 20-21°C. Dissolved oxygen levels ranged from 8.6-8.8 mg/L (97-101% air saturation). The pH ranged from 7.7-7.8 and the light intensity ranged from 354-414 lux. Test solutions were analyzed for methyl 2-hydroxyisobutyrate concentrations at test initiation and exposure termination by gas chromatography with selected ion monitoring (GC/SIM).
None of the analyses of the water control exhibited a concentration exceeding the lower limit of quantitation (LLQ) equivalent to 0.125 mg methyl 2-hydroxyisobutyrate/L. Measured concentrations of methyl 2-hydroxyisobutyrate at the limit test level ranged from 82.1 to 92.6% of the nominal concentration over the course of the exposure period. The resulting mean measured concentration was 87.4 mg methyl 2-hydroxyisobutyrate/L, equating to 87.4% of the nominal concentration. Because the mean measured concentration was within ± 20% of the nominal target concentration, results were based on the nominal (limit) concentration.
The acute toxicity values for daphnid (D. magna) exposed to methyl 2-hydroxyisobutyrate over a 48-hour exposure period and based on nominal concentrations were as follows: 24- and 48-hour EC50 : >100 mg/L (limit concentration tested); 48-hour NOEC: 100 mg/L (limit concentration tested)
Toxicity to algae
To assess potential effects of methyl 2-hydroxyisobutyrate to the freshwater green alga, Pseudokirchneriella subcapitata, an Alga Growth Inhibition Test according to OECD Testing Guideline 201 was performed for 72 hours with target concentrations of 0 (AAP control), 1.00, 3.10, 9.80, 31.3 and 100 mg methyl 2-hydroxyisobutyrate/L. Cell density was determined at approximately 24, 48 and 72 hours (±1 hour from test initiation). Temperatures during the exposure period ranged from 22-23°C. The pH ranged from 7.3-7.8 and the light intensity ranged from 4680-5520 lux. Test solutions were analyzed for methyl 2-hydroxyisobutyrate concentrations at test initiation and exposure termination by gas chromatography with selected ion monitoring (GC/SIM). None of the analyses of the AAP medium control exhibited a concentration exceeding the lower limit of quantitation (LLQ) equivalent to 0.125 mg methyl 2-hydroxyisobutyrate/L. Measured concentrations of methyl 2-hydroxyisobutyrate ranged from 83.2 to 104% of nominal concentrations over the course of the exposure period. The resulting mean measured test concentrations were 0.959, 2.99, 8.92, 28.7 and 91.2 mg methyl 2-hydroxyisobutyrate/L. Because the mean measured concentration were within ± 20% of the target nominal concentrations, study endpoints were based on nominal concentrations.
The acute toxicity values for Pseudokirchneriella subcapitata exposed to methyl 2-hydroxyisobutyrate over a 72-hour static exposure period and based on nominal concentrations were as follows:
Growth rate
72-hour ErC50: >100 mg/L (highest concentration tested)
72-hour ErC20: 95.6 mg/L (95% C.I. = 84.1 – 109 mg/L)
72-hour ErC10: 45.4 mg/L (95% C.I. = 36.1 – 57.1 mg/L)
72 -hour NOEC: 9.80 mg/L
Cell yield
72-hour EyC50: 54.8 mg/L (95% C.I. = 44.1 – 68.2 mg/L)
72-hour EyC20: 20.8 mg/L (95% C.I. = 13.5 – 32.1 mg/L)
72-hour EyC10: 12.5 mg/L (95% C.I. = 7.03 – 22.3 mg/L)
72-hour NOEC: 9.80 mg/L
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