Alcohols, C20-22, reaction products with phosphorus oxide (P2O5)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: of the alcohol 70-75°C
- Boiling point: >372°C
- Vapour pressure: 6.66 x 10-5 Pa.
- Water solubility (under test conditions): <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not ready biodegradable

Analytical monitoring:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: special solubilisation method was carried out before the test according to the OECD 203 guidance document on aquatic toxicity testing difficult substances and mixtures

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: danio rerio (Hamilton Buchanan)
- Strain: zebra Fish
- Length at study initiation (length definition, mean, range and SD): 25-30mm

- Feeding during test
- Food type: no feed



ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): in the dilution water

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

observation of the mouvement and if the caudal pedencles produce reaction after touching

Hardness:

240 mg/L of Caco3

Test temperature:

21.6 to 23.7°C

pH:

7.1 to 7.8

Dissolved oxygen:

76 to 96 %

Nominal and measured concentrations:

1;10;100mg/L

Details on test conditions:

TEST SYSTEM


- Renewal rate of test solution (frequency/flow rate):every 24h
- No. of organisms per vessel: 7 fishes
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):1
- Biomass loading rate:1,2g/L



OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:14hours



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : observation of the mouvement and the reaction of the caudal pedencles


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight: none
- Other biological observations: no signs of stress after 96h of test
- Mortality of control: none
- Other adverse effects control:none
- Abnormal responses:none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:none

Results with reference substance (positive control):

- Results with reference substance valid?
- Mortality: none
- LC50 (24h) =220mg/L
- Other:in good accordance with the historical data

Reported statistics and error estimates:

none

Sublethal observations / clinical signs:

 

Acute toxicity of the test item LCE07105 was performed on the Zebra fish Danio Rerio, under semi static conditions (test solutions are renewed every 24 hours) and according to the OECD 203 guideline. The result obtained on fishes lethality gives an LC50 -96hours superior to the highest tested concentration, i.e 100mg/L.

This result espressed in mg/L of the nominal concentration shows that no toxicity was observed in the concentrations selected in this test on the Zebra Fish Danio Rerio. No sign of stress was observed on the fishes during 96 hours of the experiment.

In order to control and to confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution contained in the aquarium was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study (as the test was performed with semi static condition, the final sample was aged of 24 hours instead of 96 hours).

Results are presented as follow:

Nominal test Item concentrations in mg/L	totol [P]
	at 0h	at 96h
Filtered control		0.028mg/L
100	0.048 mg/L	0.095mg/L

 

Validity criteria fulfilled:

yes

Conclusions:

LC50-96hours of the test item "LCE07105" on danio rerio Zebra fish is superior to 100mg/L , value expressed in nominal concentration.

Executive summary:

Acute toxicity of the test item LCE07105 was performed on the Zebra fish Danio Rerio, under semi static conditions (test solutions are renewed every 24 hours) and according to the OECD 203 guideline. The result obtained on fishes lethality gives an LC50 -96hours superior to the highest tested concentration, i.e 100mg/L.

This result espressed in mg/L of the nominal concentration shows that no toxicity was observed in the concentrations selected in this test on the Zebra Fish Danio Rerio. No sign of stress was observed on the fishes during 96 hours of the experiment.

In order to control and to confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution contained in the aquarium was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study (as the test was performed with semi static condition, the final sample was aged of 24 hours instead of 96 hours).

Results are presented as follow:

Nominal test Item concentrations in mg/L	totol [P]
	at 0h	at 96h
Filtered control	<LOD	<0.028mg/L
100	0.048 mg/L	0.095mg/L

Description of key information

Acute toxicity of the registered substance was performed on the Zebra fish Danio Rerio, under semi static conditions (test solutions are renewed every 24 hours) and according to the OECD 203 guideline. The result obtained on fishes lethality gives an LC50 -96hours superior to the highest tested concentration, i.e 100mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L