Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 11 - 14, 2013

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: OECD N 23 Guidance Document on testing of difficult substances end mixtures (2000).

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

EC Number:

938-980-5

IUPAC Name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item identification : Soybean (Glycine max) dry purified extract
Batch number : 30432/M1
Purity : 100%

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

72 h

Post exposure observation period:

72 hours

Test conditions

Test temperature:

the incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 24.0 – 24.1 °C with a mean value of 24.0 °C and a standard deviation of 0.02 °C. The temperature range provided by OECD guideline is 24 ± 2 C. The temperature values during the test were within the provided range.

pH:

The pH values of test media were in the range of 7.74 – 7.90 at the beginning of the test period and 8.80 – 9.58 at the end. After 72 hours the pH were in the range 8.86 - 9.32.

Nominal and measured concentrations:

70.0 mg/l was founded to be the maximum soluble concentration. Therefore the final test included the following concentration: 3.0, 6.6, 14.5, 31.8, 70.0 mg/l.

Details on test conditions:

Light intensity: 5183 - 5320 Lux

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenole pestanal analytical std

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

62.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 58.7 – 71.7

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

51.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Yield

Remarks on result:

other: 50.6 – 51.7

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

31.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Any other information on results incl. tables

The algal growth rate after 72 hours of exposure was inhibited by a vale of 61.1% for the highest tested concentrations, 70.0 mg/l, compared to the negative control. For the other tested concentrations, growth rate was higher than the negative control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of test item Soybean (Glycine max) dry purified extract was tested on Pseudokirchneriella subcapitata. The 72-hour EC50 is 62.9 mg/L (growth rate) and NOEC is 31.8 mg/l (growth rate).