Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 06 - July 04, 2013

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

EC Number:

938-980-5

IUPAC Name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item identification : Soybean (Glycine max) dry purified extract
Batch number : 30432/M1
Internal number : 2912125-001

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The study was performed using aerobic activated sludge from “BRIANZACQUE” civil wastewater treatment plant. Immediately after the arrival of the sludge inoculum in the laboratory, it was put under constant aeration, in dark condition at about 20 °C ± 2.0 °C. This controlled condition were kept for 2 day. Before the start of the test the activated sludge inoculum was filtered and diluted in culture medium to get a final concentration of 4 mL/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The tested concentration of test item was of 5.0 mg/L.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

48.6

Sampling time:

7 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

60.1

Sampling time:

14 d

Remarks on result:

other: Corrected values due to nitrification (after 14 the value of BOD without nitrification was 1.01 while the biodegradation value without nitrification was 64.4%)

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

67.4

Sampling time:

28 d

Remarks on result:

other: ***Corrected values due to nitrification (after 28 the value of BOD without nitrification was 1.12 while the biodegradation value without nitrification was 72.0%)

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

70

Sampling time:

21 d

Details on results:

After 14 days the oxygen amount used in oxidising ammonium process was determined to be equal to 0.34 mg O2/L for nitrate formation while at the test end was equal to 0.36 mg O2/L for nitrite formation. Therefore, the biodegradation value obtained after 14 and 28 days of exposure (respectively
64.4% and 72.0%, mean value between two test item replicates) was corrected considering
the oxygen uptake due to nitrification.
The obtained biodegradation value after 14 days and 28 days of incubation was calculated to be equal respectively to 60.1% and 67.4% (mean value between two test item replicates).

BOD5 / COD results

BOD5 / COD

Parameter:

COD

Value:

1.56 mg O2/g test mat.

Results with reference substance:

COD = 1.67 mg O2/mg
The reference item reached the biodegradation pass level (60%) after 14 days showing that the test conditions and the inoculum were appropriate for the use in the study.
Moreover, the test item did not show any inhibitory effect on the microorganism activity at the tested concentration as demonstrated by reaching the 25% of biodegradation in the toxicity control at the 7th day of test period.

Any other information on results incl. tables

The 14-day window started after 7 days of incubation with 48.6% of biodegradation and finished at 21th day; during this period the test item showed a maximum biodegradation of 70.0% (mean percentage values between two test item replicates).

According to OECD Guideline No. 301, nitrite and nitrate produced amount at the test start

and after 7, 14, 21, 28 days of exposure was measured and the oxygen consumed amount

was calculated (Table 4, section “Evaluations”). The nitrification process occurred only at 14th

and 28th day of exposure.

After 14 days the oxygen amount used in oxidising ammonium process was determined to be

equal to 0.34 mg O2/L for nitrate formation while at the test end was equal to 0.36 mg O2/L for

nitrite formation (Table 4, section “Evaluations”).

Therefore, the biodegradation value obtained after 14 and 28 days of exposure (respectively

64.4% and 72.0%, mean value between two test item replicates) was corrected considering

the oxygen uptake due to nitrification.

The obtained biodegradation value after 14 days and 28 days of incubation was calculated to

be equal respectively to 60.1% and 67.4% (mean value between two test item replicates).

The test item, Soybean (Glycine max) dry purified extract, was found to be ready

biodegradable, having exceeded the pass level (60%) within the 14-days window (70.0%,

mean percentage value between two test item replicates).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item Soybean (Glycine max) dry purified extract has showed to be ready biodegradable under the conditions applied in a Closed Bottle method, having reached the pass level (60% biodegradation) within the 14-day window (70.0% of biodegradation reached after 21 days of incubation).
However, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the 25% of biodegradation exceeded in the toxicity control after 7 days from the test start (32.3 % of biodegradation)