D-Xylopyranose, oligomeric, 2-octyldodecyl xylosides

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2006-12-15 to 2006-12-21

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted July 17th 1992

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

*Specification:
Batch no. 1137JG
Appearance: brown paste
Composition: Octyldodecyl xyloside & Octyldodecanol
Stability in water 96h
Solubility in water < 0.1 g/l
Production date: October 2006
Expiry date: October 2007
Storage: room temperature 20 + or - 5°C
Hazard onformation: non as known

*Preparation:
As the solubility lies below 100 mg/L, the water accommodated fraction was prepared for the test. This was done by weighing the nominal local, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was membrane filtrated (pore diameter 0.45 µm).

Analytical monitoring:

no

Details on sampling:

As the solubility lies below 100 mg/L, the water accommodated fraction was prepared for the test. This was done by weighing the nominal local, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was membrane filtrated (pore diameter 0.45 µm).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

*Specification:
Species: Danio rerio
Variety: HAMILTON-BUCHANAN
Age: sexually immature young fish, lenght 2 + or - 1 cm

*Origin:
The animals were obtained from a commecial supplier. Before being used for the test, the fish were kept for 14 days under test conditions. During this period, mortality didn't sur-pass 5%.
Supplier: Weiler's Pet's best, Turmstr. 6, 67433 Neustadt
Date of arrival: July 07th, 2006

*Holding:
Danio rerio is routinely used for toxicity test. The test fish are kept following SOP 115 005 04 in the current edition.
Vessels: polyethylene aquaria
Medium: chlorine-free tap water, full composition see annex
Feeding: three times a day with warmwater fishfood and daphnia totalling to about 1-2% of body weight per day.
Medium renewal: twice a week
Photo period: 12/12 hours, using neon tubes
Temperature: 23 + or - 1°C

Test type:

static

Water media type:

other: chlorine-free tap water

Total exposure duration:

96 h

Test temperature:

23 + or - 1°C

pH:

between 7.6 and 7.8

Dissolved oxygen:

between 8.8 and 9.5 mg/l

Details on test conditions:

*General conditions:
Treatment: 100 mg/L
Duration: 96 hours
Loading: 1 fish/L
Vessels: glass aquaria, maximal volume 10 L
Aeration: accomplished with glass tubes, frequency of bubbles 1/s
Feeding: none
Photo period: 12/12 hours using neon tube
Temperature: 23 + or - 1°C
pH adjustment: none
Replicates: one vessel, each containing 7 L test solution and 7 fish
Control: one vessel, containing 7 L dilution water and 7 fish
Observations were made every 24 hours, measuring pH and O2-concentration of the old and new test solution in each vessel and documentating mortalities or abnormal behaviour.
A fish was considered dead, if no visible movement could be observed, and if touching of the caudal peduncle produced non reaction.

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

The mortalities were recorded daily and can be found in the following table.

		Dead fish	Dead fish	Dead fish	Dead fish	Dead fish	Dead fish	Dead fish	Dead fish
		24h	24h	48h	48h	72h	72h	96h	96h
Nom. Conc. in mg/L	Total of fish used	new	cum.	new	cum.	new	cum.	new	cum.
0	7	0	0	0	0	0	0	0	0
100	7	0	0	0	0	0	0	0	0

No abnormal behaviour of the surviving fish could bo observed.

Validity criteria fulfilled:

yes

Conclusions:

*Validity:
- The mortality in the control may not exceed 10% at the end of the test.
No mortality occurred in the control.
- The dissolved oxygen concentration must be at least 60% throughout the test.
The concentration of dissolved oxygen stayed above 8.8 mg/L or 100% throughout the test (100% at 101300 Pa equals 8.57 mg/L, following DIN 38408 part 22).

*Discusion:
All validity criteria were met.
The test solutions were shaken for 24 hours to guarantee that the saturation concentation of the test item had been reached.
As it was not possible to filtrate a sufficient amount of the test solution through membrane filters, a aper filter was used for the preparation of the test solution.
The result of the testcan be considered valid.

Executive summary:

As the test item is poorly soluble in water, the water-accommodated fraction was tested. This was done by shaking the nomial load with the appropriate amount of drinking water for 24 hours, followed by membrane filtration.

The test was performed as a limit test using the concentration 100 mg/L. For each cconcentration, seven fish were exposed to teh test item for 96 hours.

In the treatment and the control, no fish showed any signs of morbidity at the end of the test. The pH and oxygen value of the water were normal.

The followinf results for the test item APX 20 P were determined:

24 - 96h NOEC = 100 mg/L

24 - 96h LC50> 100 mg/L

24 - 96h LC100 > 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L