Cyclododeca-1,5,9-triene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclododeca-1,5,9-triene

EC Number:

225-533-8

EC Name:

Cyclododeca-1,5,9-triene

Cas Number:

4904-61-4

Molecular formula:

C12 H18

IUPAC Name:

cyclododeca-1,5,9-triene

Details on test material:

1,5,9-cyclododecatriene of Hüls AG, purity > 99.5%

Test animals

Species:

rabbit

Strain:

other: Small white Russian, Chbb

Details on test animals or test system and environmental conditions:

TEST ANIMALS: 
- Strain: Small white Russian, Chbb-SPF
- Sex: male and female
- Source: Dr. Karl Thomae GmbH, Biberach (Germany)
- Weight at study initiation: 2.2 - 2.7 kg
- Number of animals: 3 males, 3 females
- Feed: K4 complete feed for rabbits (Ssniff, Soest, Germany);
- Water: tap water ad libitum
Environmental conditions:
- Room temperature: 20°C (+/- 1°C);
- Humidity: 60% (+/- 5%);
- Air change: 15 times/hour;
- Light: 12 hour light/dark rhythm;

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Amount / concentration applied:

undiluted

Duration of treatment / exposure:

4 hour(s)

Observation period:

1, 24, 48, 72 hours; 6, 8, 11, 14 days after exposure

Number of animals:

6

Details on study design:

ADMINISTRATION/EXPOSURE 
- Area of exposure: ca. 6 cm2
- Occlusion: mull patch, polyethylene film, elastic dressing 
- Postexposure period: 14 days
- Removal of test substance: dist. water
EXAMINATIONS
- Scoring system: OECD; evaluation: VCI notes on safety data sheet
- Examination time points: 1, 24, 48, 72 hours; 6, 8, 11, 14 days;  evaluation based on readings 1-4

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24/48/72 h

Score:

1.2

Max. score:

8

Remarks on result:

other: additional time point after 1 h

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

1

Max. score:

4

Results 3

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0.61

Max. score:

4

Irritant / corrosive response data:

AVERAGE SCORE
- Erythema: 1.00
- Edema: 0.61

Other effects:

no other effects

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the conditions of the study, the test item 1,5,9 -cyclododecatrien showed a slightly irritant effect on the skin of male and female rabbits which leads to no classification according to 67/548/EEC and according CLP regulation (1272/2008).

Executive summary:

To test the acute skin irritant effect the test item 1,5,9 -cyclododecatrien was applied to the shaven dorsal skin of 3 male and 3 female rabbits. The product was administered undiluted and the time of exposure in the patch test was 4 hours. According to the study, the test item 1,5,9 -cyclododecatrien showed a slightly irritant effect on the skin of male and female rabbits

.