Hydroxycyclohexyl phenyl ketone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Great waterflea
- Source: Originally obtained in 1978 from Institut National de recherche Chimique Appliquée, France. Since that date in-house cultured and bred
- Age at study initiation (mean and range, SD): < 24 hours (3rd brood progeny)
- Method of breeding: synthetic fresh water according to OECD 202, photoperiod: 16 hours light : 8 hours dark, 20 ± 1 °C culture temperature
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.48 mM

Test temperature:

19.5 - 19.8 °C

pH:

7.5 - 7.7

Dissolved oxygen:

8.6 - 9.0 mg/L

Nominal and measured concentrations:

nominal: control, 4.6, 10, 22, 46, 100 mg/L
mean measured: -, 4.5, 9.9, 22.2, 47, 98.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Material, size, headspace, fill volume: glass, 50 mL fill volume
- Renewal rate of test solution (frequency/flow rate): renewal period was 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 (OECD 202)
- Alkalinity: acid capacity up to pH 4.3: 0.8 - 1 mM
- Ca/Mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: at 0 and 24 hours for initial test solution and 24 and 48 hours for renwed test medium

OTHER TEST CONDITIONS
- Photoperiod: without illumination

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

53.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

47 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

99 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50 (24 h): 1.1 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of the test material to Daphnia magna was determined to be 53.9 mg/l under the conditions of the test.

Executive summary:

In this guideline (OECD 202) study conducted with GLP certification, the 48 hour EC50 of the test material to Daphnia magna was determined to be 53.9 mg/l. The test was conducted under static conditions with test concentrations between 46 and 100 mg/l. The study result does not trigger classification as hazardous for the aquatic environment under the the conditions of the Classification, Labelling, and Packaging (CLP) regulation (1272/2008), as the test material is readily biodegradable and has a BCF <500.

Description of key information

 Study carried out according to guideline and with GLP compliance.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

53.9 mg/L

Additional information

In a semi-static test conducted according to OECD 202 guideline including analytical monitoring (BASF SE, 2010) an EC50 of 53.9 mg/L after 48 h based on mean measured concentration has been determined.