p-vinylphenol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06.12.2018 to 12.01.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: OECD Guidance Document, No. 23, September 2000

Version / remarks:

Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Maruzen Petrochemical Co., LTD. 7706RXD
- Expiration date of the lot/batch: No Data
- Purity test date: Mo Data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test was stored by freezing in a dark storage place.
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle: The infrared spectrum of the test item after the completion of the experiment was the same as that before the start of the experiment and it indicated that the test was stable under storage conditons.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Analytical monitoring:

not specified

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain:
- Source (laboratory, culture collection): American Type Culture Collection
- Age of inoculum (at test initiation):
- Method of cultivation:

ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

other: Incubation with rotary shaking

Water media type:

not specified

Total exposure duration:

72 h

Test temperature:

21-24°C

Details on test conditions:

TEST SYSTEM
- Test vessel: Sterilized 300mL Erlenmeyer flask (with gas-permeable Silicosen)
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density:
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):

GROWTH MEDIUM
- Standard medium used: yes/no
- Detailed composition if non-standard medium was used:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod:
- Light intensity and quality:
- Salinity (for marine algae):

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

13.5 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.25 mg/L

Conclusions:

The study found that EC50 was 13.5 mg/L and NOEC was 1.25 mg/L.

Description of key information

The study found that EC50 was 13.5 mg/L and NOEC was 1.25 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

13.5 mg/L

EC10 or NOEC for freshwater algae:

1.25 mg/L