Isopentyl phenethyl ether

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 March to 5 April 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Isopentyl phenethyl ether

EC Number:

259-943-3

EC Name:

Isopentyl phenethyl ether

Cas Number:

56011-02-0

Molecular formula:

C13H20O

IUPAC Name:

isopentyl phenethyl ether

Specific details on test material used for the study:

Test material name (as stated in the report): ANTHER
Appearance: Colourless liquid
Batch S12 884 T5

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 1538

Metabolic activation:

with and without

Metabolic activation system:

S-9 liver

Test concentrations with justification for top dose:

based on the dose range finding test:
(1) 500, 150, 50, 15 and 5 microg/plate
(2) 10, 5, 2.5 microg/plate

Vehicle / solvent:

Solvent: Dimethylsulphoxide

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

no

True negative controls:

no

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Remarks:

10 microg/plate for strains TA1538 and TA 98

Controls 2

Untreated negative controls:

no

Negative solvent / vehicle controls:

no

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

20 microg/plate for strains TA1537

Controls 3

Untreated negative controls:

no

Negative solvent / vehicle controls:

no

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: sodium azide

Remarks:

5 microg/plate for strains TA1535 and TA100

Results and discussion

Test results

Key result

Species / strain:

other: TA1535, TA1537. TA1538, TA98 and TA100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

Anther was toxic in all strain at the top dose 5000 microg/plate in the dose range finding test

Positive controls validity:

valid

Any other information on results incl. tables

Anther was toxic towards all of the strains at the 5000 microg/plate dose levels. Therefore the 500 microg/plate was chosen as the top dose level in the mutation test.

A slight increase in revertant colony numbers was observed with the tester strain TA98 at the lower dose levels in the presence of s9mix. No substantial increase in revertant colony numbers was observed in the repat test indicating that the original increase was spurious. No substantial increase in the revertant colony numbers of any of the remaining four strains were observed following treatment with Anther at any dose level, either in presence or absence of liver microsomal fraction (s9mix).

Applicant's summary and conclusion

Conclusions:

It is concluded that no evidence of mutagenic potential of Anther was obtained in this bacterial test system at the dose levels used.

Executive summary:

The Anther was examined for mutagenic potential in this Ames metabolic activation test to assess the potential mutagenic effect dated on 28 May 1982, performed at Huntington Laboratory.

Anther was toxic towards all of the strains at the 5000 microg/plate dose levels. Therefore the 500 microg/plate was chosen as the top dose level in the mutation test.

A slight increase in revertant colony numbers was observed with the tester strain TA98 at the lower dose levels in the presence of s9mix. No substantial increase in revertant colony numbers was observed in the repat test indicating that the original increase was spurious. No substantial increase in the revertant colony numbers of any of the remaining four strains were observed following treatment with Anther at any dose level, either in presence or absence of liver microsomal fraction (s9mix). It is concluded that no evidence of mutagenic potential of Anther was obtained in this bacterial test system at the dose levels used.