Isopentyl phenethyl ether

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 March to 03 March 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

No analysis of the test substance.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Isopentyl phenethyl ether

EC Number:

259-943-3

EC Name:

Isopentyl phenethyl ether

Cas Number:

56011-02-0

Molecular formula:

C13H20O

IUPAC Name:

isopentyl phenethyl ether

Specific details on test material used for the study:

Test material name (as stated in the report): ANTHER
Appearance: Clear colourless liquid
Sample Code: N54
Expiry date: 31 December 1994

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

There was no analysis of the test compound during this test.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna were cultured in the laboratory. Cultures were maintained in a synthetic medium with the same chemical composition and similar pH dissolved oxygen concentrations as that used to prepare the test solutions.
The cultures are fed daily with cells of the green algae Chlorella vulgaris. Cultures are reset using 10 neonates, when they become gravid their numbers are reduced from 10 to 5 and the production of neoantes results in their daily removal.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

233 mg/l as CaCO3

Test temperature:

20.4°C

pH:

7.8

Dissolved oxygen:

8.3 mg/L

Nominal and measured concentrations:

Nominal concentration: 0.0; 0.56; 1.0; 1.8; 3.2; 5.6 and 10.0 mg/L

Details on test conditions:

Daphnia were exposed to a range of concentrations 0.56 - 10 mg/L. The test was conducted under static renewal conditions with test media renewal at 24 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits (3.2 - 10 mg/L)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

At 48 hours Daphnia exposed to 1.8 mg/L of the test substance and above were not swimming freely. Movement was spasmodic and only observed at the bottom of the test vessels. At a concentration of 1.0 mg/L and below, this abnormal behaviour was not observed.
The % immobile at 48 hours is reported as 0, 10, 0, 0, 0, 60 and 100% at respectively nominal concentrations of 0, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48 hours) has been determined to be 5.2 mg/L with 95% confidence limits of 3.2 - 10 mg/L and the NOEC (48 hours) NOEC (48 hours) was determined to be 1.0 mg/L in this test conditions.

Executive summary:

The acute toxicity of Anther to the freshwater cladoceran Daphnia Magna was determined according to the OECD Guideline 202. The test was conducted under static renewal conditions with test media renewal at 24 hours. Daphnia were exposed to a range of concentrations 0.56 - 10.0 mg/L for a total period of 48 hours. There was no analysis of the test substance during the test period but from its chemical structure the substance is expected to be hydrolytically stable. The EC50s based on nominal concentrations and calculated by the Binomial method are : EC50 (24 hours) > 10mg/L; EC50 (48 hours) = 5.2 mg/L with 95% confidence limits of 3.2 - 10 mg/L. The corresponding NOECs are 5.6 and 1.0mg/L respectively.

At 48 hours, Daphnia in the higher concentrations were classed as mobile but it was considered that these were affected by the test substance since they were observed moving through the lower levels of the medium only in a spasmodic fashion. At a concentration of 1.0 mg/L and below, this abnormal behaviour was not observed.