Isopentyl phenethyl ether

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 February to 4 March 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Principles of method if other than guideline:

There were no analysis of the test substance concentrations in the test medium in this test.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isopentyl phenethyl ether

EC Number:

259-943-3

EC Name:

Isopentyl phenethyl ether

Cas Number:

56011-02-0

Molecular formula:

C13H20O

IUPAC Name:

isopentyl phenethyl ether

Specific details on test material used for the study:

Test material name (as stated in the report): ANTHER
Appearance: Clear colourless liquid
Sample Code: N54
Storage: room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

0.208g of Anther was dispersed with high energy sonication in 1.0 liter of distilled water to give a nominal 200 mg/L stock. A range of test solution (nominally 0.32 - 32 mg/L) was prepared by diluting the stock with ISO algal medium.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Test conditions

Test temperature:

20.5 - 22.5 °C

pH:

Test solution pHs at 0 hours : 7.4 - 7.7
Test solution pHs at end of the test: 7.6 - 7.8

Nominal and measured concentrations:

Nominal concentration 0.32; 1.0; 3.2; 10 and 32 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

Effects based on biomass were also reported (see attached full study report). However, the preferred observational endpoint in the algal inhibition study is growth rate because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. Thus only the effects based on growth rate are presented in the above "effects concentration" table.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72 hour EC50 (growth rate) has been determined to be > 32 mg/L based on nominal concentrations. The 72 hour NOEC was 10 mg/L.

Executive summary:

The toxicity of Anther to the Freshwater green Algal Scenedesmus subspicatus was determined in compliance with OECD standard method. A range of concentrations of the test substance (nominallly 0.32 to 32 mg/L) was prepared in ISO medium. Based on nominal concentrations, the ErC50 (72 hours) has been determined to be > 32 mg/L in this test conditions. The 72 hour NOEC was 10 mg/L.