Registration Dossier
Registration Dossier
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EC number: 613-004-8 | CAS number: 62285-89-6
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
Test material
- Reference substance name:
- [(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride
- EC Number:
- 613-004-8
- Cas Number:
- 62285-89-6
- Molecular formula:
- C28 H32 O2 P . Cl
- IUPAC Name:
- [(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride
- Details on test material:
- - Name of test material (as cited in study report): C5-NPG-Acetal
- Analytical purity: not stated
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared (2 g/l)
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Initial biomass concentration: 1 g/l dw
Study design
- Total exposure duration:
- 30 min
Test conditions
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
1,
4,
8,
10,
100,
400,
1000,
control - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorphenole
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC20 ca.6 mg/l,
EC50 ca.45 mg/l,
EC80 ca.250 mg/l
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
