[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

EC Number:

613-004-8

Cas Number:

62285-89-6

Molecular formula:

C28 H32 O2 P . Cl

IUPAC Name:

[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

Details on test material:

- Name of test material (as cited in study report): C5-NPG-Acetal
- Analytical purity: 95%
- Lot/batch No.: 92/33-1
- Physical state: solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared separately.
- Differential loading: yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West aquarium GmbH, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 5 month
- Length at study initiation (length definition, mean, range and SD): 2.59 cm (range 2.4 - 2.8 cm)
- Weight at study initiation (mean and range, SD): 0.19 g (range 0.14 - 0.26 g)

Study design

Test type:

static

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

23 - 24 °C

pH:

8.2 - 8.4

Dissolved oxygen:

6.9 - 7.9 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
50,
100,
control

Measured test concentrations:
after 1 h:
50.1 mg/l
100.8 mg/l
after 96 h:
49.2 mg/l
98.1 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30x22x24 cm)
- Type (delete if not applicable): open
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.2 g/l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city Frankenthal (Germany), not chlorinated.


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality after 1, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Range finding study:
- Results used to determine the conditions for the definitive study: no toxic signs up to a concentration of 100 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

Finley, probit analysis