Registration Dossier
Registration Dossier
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EC number: 613-004-8 | CAS number: 62285-89-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, however adapted sludge was used
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- [(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride
- EC Number:
- 613-004-8
- Cas Number:
- 62285-89-6
- Molecular formula:
- C28 H32 O2 P . Cl
- IUPAC Name:
- [(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride
- Details on test material:
- - Name of test material (as cited in study report): C5-NPG-Acetal
- Physical state: solid
- Lot/batch No.: 92/33-1 (Technikum B 155)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge of BASF waste water treatment plant mixed with activated sludge of a laboratory waste water treatment plant.
- Pretreatment: Adaption of activated sludge according to SOP M 63/1 (14 days) - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 14.2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 500 ml water, 6 ml nutrient solution, 0.5 ml inoculum, 14.2 mg/l test substance, filled up with water to a total volume of 1000 ml
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Measuring equipment: Shimadzu
SAMPLING
- Sampling frequency: daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: Reference substance: 1
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- < 10
- Sampling time:
- 28 d
Applicant's summary and conclusion
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