[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 79/831/EEC, Annex V, Part C

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

EC Number:

613-004-8

Cas Number:

62285-89-6

Molecular formula:

C28 H32 O2 P . Cl

IUPAC Name:

[(2E)-3-(5,5-dimethyl-1,3-dioxan-2-yl)-2-butenyl]triphenylphosphonium chloride

Details on test material:

- Name of test material (as cited in study report): C5-NPG-Acetal
- Analytical purity: > 99 %
- Lot/batch No.: 92/33-1
- Physical state: solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution was prepared by suspending a defined amount of test substance in the test medium (100 mg/l). By dilution of the stock solution with test medium the test concentrations were prepared separately.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.42 mmol/l

Test temperature:

19.3 - 20.6 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.2 - 8.4 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.09,
0.19,
0.39,
0.78,
1.56,
3.13,
6.25,
12.5,
25,
control

Measured test concentrations (mg/l):
1.67 (nominal = 1.56),
6.46 (nominal = 6.25),
25.29 (nominal = 25),
98.95 (nominal = 100)

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1/2 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic M4 medium
- Alkalinity: up to pH 4.3 = 0.89 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 603 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 day-night regime
- Light intensity: 5 - 6 µE/(m²s) in the range of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.78 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility