Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: New testing design followed (in line with OECD TG 401). No individual data, limited reporting of experimental details

Data source

Reference
Reference Type:
publication
Title:
A new approach to practical acute toxicity testing.
Author:
Lorke D.
Year:
1983
Bibliographic source:
Arch Toxicol., vol. 54, no. 4, p. 275-87.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
- this survey study was conducted to evaluate a new strategy for determination of LD50 values with a lower number of animals
- nevertheless in total 11 animals were used for each dose group
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon tetrachloride
EC Number:
200-262-8
EC Name:
Carbon tetrachloride
Cas Number:
56-23-5
Molecular formula:
CCl4
IUPAC Name:
tetrachloromethane

Test animals

Species:
rat
Strain:
not specified
Sex:
male
Details on test animals or test system and environmental conditions:
- Acclimation period: 5 d
- no further details reported

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Details on oral exposure:
- no details reported
Doses:
1500, 2000, 2800, 3900 mg/kg bw
No. of animals per sex per dose:
in total 11
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days or longer until the surviving animals stated gaining weight again
- Frequency of observations and weighing: days 0, 7, 14 and weekly thereafter if necessary
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs daily
Statistics:
Method of Rosiello et al. (RosieUo AP, Essigmana JM, Wogan GN (1977) Rapid and accurate determination of the median lethal dose (LDSo) and its error with a smalI computer. J Toxicol Environm Health 3: 797-809), based on the method by Bliss (1938).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
2 500 mg/kg bw
Mortality:
1500 mg/kg bw 0/11
2000 mg/kg bw 5/11
2800 mg/kg bw 6/11
3900 mg/kg bw 11/11
Clinical signs:
other: - not reported
Gross pathology:
- not reported

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: other: According to EU directive 67/548/EEC and EU RegAccording to EU directive 67/548/EEC and EU Regulation (EC) N0. 1272/2008 (CLP)
Conclusions:
The present study (Lorke, 1983) states a LD50 (rat, oral) of 2500 mg/kg bw after single oral application via gavage.
Executive summary:

The potential of the test substance CTC (carbon tetrachloride) to induce toxicity upon exposure via the oral route was evaluated in a survey study on different substances following generally OECD TG 401. The scope of the study was to evaluate a new strategy to determine LD50 values with a lower amount of animals. Nevertheless the total amount of tested animals in each dose group allowed for CTC a classical determination of a LD50 value according to OECD TG 401. Male rats (unspecified strain) were treated at a single oral dose bw with CTC (unknown vehicle). Observation period was 14 d and further experimental details were not reported but stated to be conducted according to OECD TG 401.

The mortality incidences were 0/11 at 1500 mg/kg bw, 5/11 at 2000 mg/kg, 6/11 at 2800 mg/kg and 11/11 at 3900 mg/kg.

The LD 50(rat, oral) was therefore assessed to be 2500 mg/kg bw after single oral application via gavage.