Registration Dossier
Registration Dossier
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EC number: 225-768-6 | CAS number: 5064-31-3
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well-documented publication which meets basic scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trisodium nitrilotriacetate
- EC Number:
- 225-768-6
- EC Name:
- Trisodium nitrilotriacetate
- Cas Number:
- 5064-31-3
- Molecular formula:
- C6H9NO6.3Na
- IUPAC Name:
- trisodium 2-[bis(carboxylatomethyl)amino]acetate
- Details on test material:
- Na3.NTA ("TRILON A"), liquid, 38 % aqueous
solution, not further specified (e.g. pH not stated)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- No. of animals per cage: single housing. The animals were housed in fully air-conditioned rooms, in which a central air-conditioning system en¬sured a temperature in the range of 20 – 24°C and a relative humidity in the range of 30 – 70%. Illumination period: 12 h dark/12 h light. Acclimatization period: about 8 days before the beginning of the study. Water: daily about 250 ml per animal.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated eye of the same animal
- Amount / concentration applied:
- 0.1 ml
- Duration of treatment / exposure:
- 4 h
- Observation period (in vivo):
- 8 days
- Number of animals or in vitro replicates:
- 3
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 7
- Max. score:
- 110
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 48 h
- Score:
- 3
- Max. score:
- 110
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 72 h
- Score:
- 1
- Max. score:
- 110
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 8 d
- Score:
- 0
- Max. score:
- 110
- Irritant / corrosive response data:
- moderate erythema, slight edema and secretion after 24 h, slight erythema still visible after 72 h on 2 animals
Any other information on results incl. tables
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
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