3-bromopropene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted in compliance with agreed protocols, with no deviations from standard test guidelines and no methodological deficiences, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

3-bromopropene

EC Number:

203-446-6

EC Name:

3-bromopropene

Cas Number:

106-95-6

Molecular formula:

C3H5Br

IUPAC Name:

3-bromoprop-1-ene

Constituent 2

Reference substance name:

1-bromoprop-2-ene

IUPAC Name:

1-bromoprop-2-ene

Details on test material:

Lot 4-258-1
Colorless liquid
Container: one smoked glass flask
Purity: 99.5 %
Acidity (HBr) < 70 mg/kg
Storage conditions: at room temperature and protected from light

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Number and sex: one group of ten animals (five males and five females).
Age/weight: on the day of treatment, the animals were approximately six weeks old, and had a mean body weight ± standard deviation of 177±6 g for the males and 155±6 g for the females.
Acclimatization: at least five days before the beginning of the study.
Identification of the animals: the animals were identified individually by earmarks or earnotches.

During the acclimatization period and during the main test, the conditions in the animal room were as follow:
- temperature: 21±2°C
- relative humidity: 30 to 70%
- light/dark cycle: 12h/12h
- ventilation: about 12 cycles/hour of filtered, non-recycled air.
The temperature and relative humidity were recorded continuously and record retained.
The housing conditions (temperature, relative humidity and ventilation) were checked monthly.

The animals were housed in polycarbonate cages (48x27x20 cm). Each cage contained four to seven animals of the same sex during acclimatization period and five rats of the same sex during the treatment period. Each cage contained dustfree sawdust.
Bacteriological analysis of the sawdust and detection of possible contaminats (pesticides, heavy metals) are performed periodically.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

Preliminary study: 2000 mg/kg bodyweight
Second step: 200 mg/kg bodyweight

No. of animals per sex per dose:

Preliminary study: 5 males and 5 females
Second step: 5 males and 5 females

Control animals:

no

Results and discussion

Preliminary study:

The result of the preliminary study indicated that the acute lethal oral dose of allyl bromide was less than 2000 mg/kg bodyweight

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

200 mg/kg bw

Based on:

test mat.

Mortality:

No death occured during the observation period

Clinical signs:

other: Hypoactivity and piloerection wer observed on all animals up to day 4. First signs were observed 1 to 2 hours after treatment. Recovery was complete on day 5. .

Gross pathology:

Adhesions between the stomach, the spleen, the liver and the abdominal wall were observed in the animals of this group at necropsy

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the experimental conditions, the oral LD50 of allyl bromide is between 200 and 2000 mg/kg in rats.
According to CLP regulation allyl bromide is classified as Toxic category III with the hazard statement H301 Toxic if swallowed.