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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study conducted in compliance with agreed protocols, with no deviations from standard test guidelines and no methodological deficiences, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
yes
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Lot 4-258-1
Colorless liquid
Container: one smoked glass flask
Purity: 99.5 %
Acidity (HBr) < 70 mg/kg
Storage conditions: at room temperature and protected from light

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
Number and sex: one group of ten animals (five males and five females).
Age/weight: on the day of treatment, the animals were approximately six weeks old, and had a mean body weight ± standard deviation of 177±6 g for the males and 155±6 g for the females.
Acclimatization: at least five days before the beginning of the study.
Identification of the animals: the animals were identified individually by earmarks or earnotches.

During the acclimatization period and during the main test, the conditions in the animal room were as follow:
- temperature: 21±2°C
- relative humidity: 30 to 70%
- light/dark cycle: 12h/12h
- ventilation: about 12 cycles/hour of filtered, non-recycled air.
The temperature and relative humidity were recorded continuously and record retained.
The housing conditions (temperature, relative humidity and ventilation) were checked monthly.

The animals were housed in polycarbonate cages (48x27x20 cm). Each cage contained four to seven animals of the same sex during acclimatization period and five rats of the same sex during the treatment period. Each cage contained dustfree sawdust.
Bacteriological analysis of the sawdust and detection of possible contaminats (pesticides, heavy metals) are performed periodically.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Doses:
Preliminary study: 2000 mg/kg bodyweight
Second step: 200 mg/kg bodyweight
No. of animals per sex per dose:
Preliminary study: 5 males and 5 females
Second step: 5 males and 5 females
Control animals:
no

Results and discussion

Preliminary study:
The result of the preliminary study indicated that the acute lethal oral dose of allyl bromide was less than 2000 mg/kg bodyweight
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
200 mg/kg bw
Based on:
test mat.
Mortality:
No death occured during the observation period
Clinical signs:
Hypoactivity and piloerection wer observed on all animals up to day 4. First signs were observed 1 to 2 hours after treatment. Recovery was complete on day 5.
.
Body weight:
Slight decrease in body weight gain was noted the first week in both sexes. Body weight gain was normal the second week.
Gross pathology:
Adhesions between the stomach, the spleen, the liver and the abdominal wall were observed in the animals of this group at necropsy

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category III
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Under the experimental conditions, the oral LD50 of allyl bromide is between 200 and 2000 mg/kg in rats.
According to CLP regulation allyl bromide is classified as Toxic category III with the hazard statement H301 Toxic if swallowed.