Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study done with old guidelines but which are similar to actual. The study was conducted with a number of important groups of petroleum-derived chemicals. The conditions of the study can be judged acceptable.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1979

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: American Public Health Association - Static tank acute toxicity test
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
No data

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Carassius auratus
Details on test organisms:
Goldfish of uniform lenght (average 6.2 ± 0.7 cm) and weight (average 3.3 ± 1.0 g) and in good health

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
24 h
Post exposure observation period:
None

Test conditions

Hardness:
Not reported
Local tap-water : alkali as Na+ = 30 mg/L
Test temperature:
20 ± 1°
pH:
Adjusted to 7.0 with NaOH or H2SO4.
Dissolved oxygen:
Due to volatility of allyl bromide the procedure had to be adapted slightly to avoid more than 10% loss. Test was done without aeration.
Test limited at 24 h to ensure that the dissolved oxygen content did not fall below 4.0 mg/L.
Salinity:
In mg/L: Cl- = 65 ; NO2- = 0 ; NO3- = 4 ; SO4 2- = 35 ; PO4 3- = 0.15 ; HCO3- = 25 ; SiO2 = 25 ; NH4+ = 0 ; Fe = 0.05 ; Mn = 0 ; Ca2+ = 100 ; Mg2+ = 8
Nominal and measured concentrations:
Not reported
The concentrations of the chemical in the solutions tested were determined before and efter each test, either with a total organic carbon analyser or by extraction and subsequent GC analysis.
Details on test conditions:
Test material was tested at a series of concentrations. In each test 6 fish were used. Solutions were contained in all-glass tanks measuring 42x 28x 28 cm.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
800 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
LC50 of allyl bromide to Goldfish has been determined to be 800 µg/L.
Executive summary:

With the guidelines laid down by the American Public Health Association for static-tank acute toxicity test, LC50 of allyl bromide to Goldfish after 24 h test has been determined to be 800 µg/L.