4-chlorobutyryl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study; E. coli not tested

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

in accordance with Ames B.N. et al.: Proc. Nat. Acad. Sci. USA, 70, 2281-2285, (1973) as well as Ames B.N. et al.: Mut. Res. 31, 347-364, (1975)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

4-chlorobutyryl chloride

EC Number:

225-059-1

EC Name:

4-chlorobutyryl chloride

Cas Number:

4635-59-0

Molecular formula:

C4H6Cl2O

IUPAC Name:

4-chlorobutanoyl chloride

Details on test material:

Name of the test substance used in the study report: 4-Chlorbuttersäurechlorid
Purity: ca. 99%

Method

Target gene:

S. typhimurium

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 1538

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254-induced rat liver S-9 fraction

Test concentrations with justification for top dose:

20 - 5000 ug/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

Positive controls:

yes

Positive control substance:

other: with S-9 mix (all strains): 2-Aminoanthracene (10 µg); without S-9 mix: N-Methyl-N'-nitro-N-nitroso-guanidine (5 µg) for strains TA 100, TA 1535 and TA 98, 4-Nitro-o-phenylenediamine (10 µg), 9-Aminoacridinium chloride monohydrate (100 µg) for TA 100

Details on test system and experimental conditions:

TEST SYSTEM
System: standard plate test.
Metabolic activation: with and without, using liver S-9 supernatant of male Aroclor 1254 induced Sprague-Dawley
rats.
Incubation: plates were incubated at 37 °C for 48 hours in the dark; the number of colonies was then counted.
Replicates: 4 plates per dose and control were used in 2 independent experiments.

Experiment 1 (standard plate test; with and without S-9 mix;
all strains): 20, 100, 500, 2500 and 5000 µg/plate;
Experiment 2 (standard plate test; with and without S-9 mix;
all strains): 250, 500, 750, and 1000 µg/plate.

Evaluation criteria:

test results considered positive, if
- doubling of revertant colonies compared to spontaneous mutation rate
- dose effect relationship
- reproducibility of results

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: > 750 ug/plate

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: > 750 ug/plate

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Cytotoxicity:
A bacteriotoxic ffect (reduced his- or trp- background growth, decrease in the number of his+ or trp+ revertants in the titer was observed from about 750 µg onwards

Solubility
No precipitation of the substance was found.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.