Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 695-022-6 | CAS number: 473278-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Remarks:
- activated sludge respiration and nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Aug - 07 Nov 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Düsseldorf, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item was weighed directly into 300 mL glass Erlenmeyer flasks to 130 mL deionised water and was stirred overnight for 17 hours. 8 mL of the synthetic medium and 100 mL of activated sludge were added. The mixture was filled up with deionised water to 250 mL and aerated at 20 ± 2 °C. The same design was performed for the determination of the heterotrophic oxidation with the addition of 1.25 mL ATU solution (2.32 g/L N-allythiourea diluted with 1 L deionised water). For the reference compound a stock solution at a concentration of 500 mg/L was prepared by dissolving 250 mg 3,5-Dichlorophenol in 5 mL of 1 N NaOH and diluting to 0.5 litre with deionised water.
- Differential loading: 10, 32, 100, 320 and 1000 mg/L. Two sets of reaction mixtures were prepared, one without ATU and one with ATU.
- Controls: yes (6 replicates for control and 6 for ATU control) - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: mixed population of aquatic microorganisms of the aeration tank of a domestic waste water treatment plant (Municipal WWTP Cologne-Stammheim), collection date: 04 Oct 2016
- Preparation of inoculum for exposure: The sludge was settled and the supernatant was decanted. After centrifuging the sludge (15 min at 3500 rpm and 20 °C) the supernatant was decanted again. Approximately 1 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry weight) suspended solids. The calculated amount of sludge was dissolved in synthetic medium and then filled up to a defined end volume with deionised water.
- Pretreatment: aeration of the activated sludge at 20 ± 2 °C, daily fed with synthetic medium
- Initial biomass concentration: 800 mg/L suspended solids (Test concentration of the activated sludge) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18.5 - 20.3 °C (controls)
18.7 - 19.8 °C (test item)
19.0 - 20.0 °C (reference item) - pH:
- 7.2 - 7.4 (adjusted at test start, test item, control)
7.0 - 7.5 (adjusted at test start, reference compound)
7.4 - 8.4 (at test end) - Nominal and measured concentrations:
- control, 10, 32, 100, 320, and 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass Erlenmeyer flasks
- Type: closed
- Material, size, headspace, fill volume: 300 mL Erlenmeyer flasks; 250 mL of sludge with the test item (or control or reference compound)
- Aeration: test system was stirred and aerated through a glass tube at 50 - 100 L/h with clean oil-free air
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 3 g (dry weight)/L
- Nutrients provided for bacteria: A synthetic waste water feed was prepared (medium according to the guideline)
- Nitrification inhibitor used: N-allylthiourea
- Biomass loading rate: 800 mg/L suspended solids (Test concentration of the activated sludge)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Particulate matter: Temperature and pH were measured at test start and test end.
OTHER TEST CONDITIONS
- Adjustment of pH: for the reference compound pH was adjusted to pH 7 ± 0.5 with HCl at test start, for test item concentrations and control pH was adjusted to pH 7.2 - 7.4 with NaOH at test start
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After the 3-hour contact time, the oxygen consumption was recorded
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
Range finding study:
- Test concentrations: 100 mg/L, 3 replicates
- Results used to determine the conditions for the definitive study: see"Any other information on material and methods incl. tables" - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- test item = act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Details on results:
- - Blank controls oxygen uptake rate: In the present test oxygen uptake rate was 53.2 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour for the test set without ATU and 46.4 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour for the test set with ATU
- Coefficient of variation of oxygen uptake rate in control replicates: 8.6% for the test set without ATU and; 3.9% for the test set with ATU
- The effect value relates to a nominal concentration, since no analytical monitoring was performed.
- The physico-chemical oxygen consumption has been determined at 1000 mg/L test item concentration. As nearly no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
- All validity criteria were met.
- Results in detail see Table 1 and 2 in "Any other information on results incl. tables" - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, the EC50 of the reference compound 3,5-Dichlorophenol was 17.8 mg/L for total respiration and 15.7 mg/L for heterotrophic respiration.
- Relevant effect levels: the EC50 was between 20- 40 mg/L - Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 in "Any other information on results incl. tables".
Reference
Table 1: Main test, Test without ATU (Total respiration). Respiration rates after 3 hours incubation period, percentage inhibition, temperature and pH values
Treatment [mg/L] |
Respiration rate |
Mean Temp. |
pH- |
Inhibition |
|
|
|
[mg/L · h] |
[°C] |
value |
[%] |
Control 1 |
|
48.341 |
20.3 |
8.3 |
-- |
Control 2 |
|
44.835 |
20.3 |
8.3 |
-- |
Control 3 |
|
42.952 |
20.1 |
8.4 |
-- |
Control 4 |
|
39.226 |
18.5 |
8.4 |
-- |
Control 5 |
|
41.466 |
18.7 |
8.3 |
-- |
Control 6 |
|
38.608 |
18.7 |
8.4 |
-- |
Control, mean |
|
42.571 |
-- |
-- |
-- |
Test item |
10 |
40.024 |
19.3 |
8.3 |
5.983 |
Test item |
10 |
39.649 |
18.8 |
8.4 |
6.864 |
Test item |
10 |
41.163 |
19.0 |
8.3 |
3.309 |
Test item |
32 |
40.938 |
18.9 |
8.4 |
3.837 |
Test item |
32 |
41.773 |
190 |
8.4 |
1.876 |
Test item |
32 |
43.749 |
19.0 |
8.4 |
0.000 |
Test item |
100 |
43.895 |
19.3 |
8.3 |
0.000 |
Test item |
100 |
40.648 |
18.8 |
8.4 |
4.518 |
Test item |
100 |
45.439 |
19.1 |
8.4 |
0.000 |
Test item |
320 |
41.796 |
19.1 |
8.3 |
1.821 |
Test item |
320 |
42.447 |
19.0 |
8.3 |
0.293 |
Test item |
320 |
40.149 |
18.9 |
8.4 |
5.690 |
Test item |
1000 |
36.331 |
19.0 |
8.4 |
14.658 |
Test item |
1000 |
41.132 |
19.0 |
8.4 |
3.382 |
Test item |
1000 |
40.169 |
18.7 |
8.4 |
5.642 |
Test item, mean |
10 |
40.279 |
-- |
-- |
5.385 |
Test item, mean |
32 |
42.153 |
-- |
-- |
1.904 |
Test item, mean |
100 |
43.327 |
-- |
-- |
1.506 |
Test item, mean |
320 |
41.464 |
-- |
-- |
2.601 |
Test item, mean |
1000 |
39.211 |
-- |
-- |
7.894 |
Physico-chemical oxygen consumption control |
1000 |
0.489 |
18.7 |
7.6 |
-- |
Reference compound |
2.5 |
41.316 |
19.7 |
8.4 |
2.948 |
Reference compound |
5 |
39.845 |
19.6 |
8.3 |
6.404 |
Reference compound |
10 |
33.479 |
20.0 |
8.3 |
21.357 |
Reference compound |
20 |
16.809 |
19.9 |
8.4 |
60.514 |
Reference compound |
40 |
8.662 |
19.5 |
8.4 |
79.654 |
Table 2: Main test, Test with ATU (Heterotrophic respiration). Respiration rates after 3 hours incubation period, percentage inhibition, temperature and pH values
Treatment [mg/L] |
Respiration rate |
Mean Temp. |
pH- |
Inhibition |
|
[mg/L · h] |
[°C] |
value |
[%] |
||
Control 1 |
|
35.717 |
19.5 |
8.2 |
-- |
Control 2 |
|
37.066 |
19.5 |
8.2 |
-- |
Control 3 |
|
35.705 |
19.1 |
8.2 |
-- |
Control 4 |
|
38.580 |
18.5 |
8.3 |
-- |
Control 5 |
|
39.119 |
18.8 |
8.3 |
-- |
Control 6 |
|
36.565 |
19.1 |
8.3 |
-- |
Control, mean |
|
37.125 |
-- |
-- |
-- |
Test item |
10 |
38.148 |
19.5 |
8.3 |
0.000 |
Test item |
10 |
34.990 |
18.8 |
8.4 |
5.753 |
Test item |
10 |
36.497 |
19.0 |
8.3 |
1.693 |
Test item |
32 |
36.728 |
19.0 |
8.3 |
1.070 |
Test item |
32 |
37.967 |
19.2 |
8.3 |
0.000 |
Test item |
32 |
37.358 |
19.2 |
8.4 |
0.000 |
Test item |
100 |
37.028 |
19.4 |
8.1 |
0.261 |
Test item |
100 |
37.237 |
19.0 |
8.3 |
0.000 |
Test item |
100 |
37.567 |
19.1 |
8.3 |
0.000 |
Test item |
320 |
44.513 |
19.8 |
8.3 |
0.000 |
Test item |
320 |
40.525 |
19.6 |
8.3 |
0.000 |
Test item |
320 |
39.761 |
19.5 |
8.4 |
0.000 |
Test item |
1000 |
38.175 |
19.2 |
8.3 |
0.000 |
Test item |
1000 |
39.572 |
19.4 |
8.4 |
0.000 |
Test item |
1000 |
38.380 |
18.8 |
8.4 |
0.000 |
Test item, mean |
10 |
36.545 |
-- |
-- |
2.482 |
Test item, mean |
32 |
37.351 |
-- |
-- |
0.357 |
Test item, mean |
100 |
37.278 |
-- |
-- |
0.087 |
Test item, mean |
320 |
41.600 |
-- |
-- |
0.000 |
Test item, mean |
1000 |
38.709 |
-- |
-- |
0.000 |
Physico-chemical oxygen consumption control 1000 |
0.724 |
18.9 |
7.4 |
-- |
|
Reference compound |
2.5 |
35.157 |
19.0 |
8.2 |
5.301 |
Reference compound |
5 |
36.849 |
19.2 |
8.2 |
0.745 |
Reference compound |
10 |
26.371 |
19.6 |
8.2 |
28.967 |
Reference compound |
20 |
11.233 |
19.4 |
8.3 |
69.744 |
Reference compound |
40 |
8.385 |
19.3 |
8.3 |
77.413 |
Table 3: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
The oxygen uptake rate was 53.2 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour for the test set without test substance and 46.4 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour for the test set with test substance. |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
The CV was 8.6% for the test set without test substance and; 3.9% for the test set with test substance. |
yes |
Description of key information
EC50 (total, heterotrophic and nitrification respiration) > 1000 mg/L (nominal) for activated sludge (OECD 209)
NOEC (total, heterotrophic and nitrification respiration) ≥ 1000 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
One key study is available for tefuryltrione, in which the toxicity to microorganisms was investigated. The study was conducted according to GLP and OECD guideline 209. The activated sludge was exposed to the test item at five test item concentrations of 10, 32, 100, 320 and 1000 mg/L for 3 h. Test concentrations are given as nominal concentrations. The respiration rate (oxygen consumption) is classified into two processes of oxidation, the oxidation of organic carbon (heterotrophic oxidation) and the ammonium oxidation (nitrification). By measuring the oxygen consumption rates in the presence and absence of a specific inhibitor of nitrification ATU (N-allylthiourea), total, heterotrophic and nitrification respiration rates were calculated. Nitrification inhibition could not be calculated as the differences between total and heterotrophic respiration rates were too small. As no inhibition was observed for total and heterotrophic respiration up to the highest tested concentration of 1000 mg/L, the same effect values for nitrification were chosen. No effect was detected up to a test item concentration of 1000 mg/L. Therefore, the EC50s and NOECs for total respiration, heterotrophic respiration, and nitrification respiration were determined as >1000 mg/L and ≥1000 mg/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.