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Diss Factsheets
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EC number: 695-022-6 | CAS number: 473278-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Jul - 03 Aug 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Düsseldorf, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: aeration tank of the wastewater treatment plant Odenthal, Germany, treating predominantly domestic sewage
- Storage conditions: at room temperature under continuous stirring with aeration
- Storage length: 2 d, i.e. date of collection: 4 Jul 2016, start of incubation: 6 Jul 2016
- Pretreatment and preparation of inoculum for exposure: One day before start of the test the sludge was washed twice by adding mineral medium and centrifuging for 10 min at 2000 rpm and 20 °C and decanting off the supernatant. An aliquot of the wet sludge was dried in order to determine the wet weight/dry weight ratio of the sludge and to prepare a stock suspension (activated sludge) of 3 g dw/L. The calculated amount of sludge, needed to achieve 300 mL of this stock suspension, was dissolved in mineral medium and then filled up to a defined end volume. The suspension was stored again at room temperature under continuous stirring with aeration until start of the study the next day. Evaporated mineral medium was replaced.
- Concentration of inoculum: 30 mg/L suspended solids - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- 1.698 other: mg O2/mg
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium was prepared from 4 stock solutions of mineral components in deionised water, namely of 1) Mineral salt solution, 2) Magnesium sulphate solution, 3) Calcium chloride solution and 4) Iron (III) chloride solution
- Test temperature: 22 ± 1 °C
- pH: 7.3 - 7.9 after 28 d
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Test apparatus: OxiTopControl System (WTW)
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: The suspension of activated sluge was continuously aerated until study start.
- Details of trap for CO2: NaOH
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes: inoculum without test item, triplicate
- Reference: yes: inoculum wtih sodium benzoate, duplicate
- Toxicity control: yes: inoculum with test item and reference compound mixed, 1 replicate - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Remarks:
- See "Any other information on results incl. tables".
- Interpretation of results:
- not readily biodegradable
Reference
The toxicity control exhibited degradation rates > 25% within 12 d. The test substance was not toxic to bacteria at the tested concentration.
The reference compound showed 69% degradation after 14 d.
Degradation of the test substance was 4% after 7 and 14 d, 6% after 21 d and 4% after 28 d. At the end of the test, at the plateau, or the end of the 10 d window, biodegradation in parallels with test item did not differ by more than 20 percentage points.
The oxygen uptake of the inoculum blank was 48.8 mg O2/L after 28 days.
The test substance was considered to be "Not Readily Biodegradable".
Description of key information
Not Readily Biodegradable (4% in 28 d, OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
One GLP guideline study is available, which assessed the "Ready Biodegradability" of the test substance according to OECD guideline 301 F. Activated sludge was collected from the aeration tank of a local WWTP treating predominantly domestic sewage and was continuously aerated until test start. The test medium with a nominal concentration of 100 mg/L test substance, equivalent to 1.698 mg O2/mg ThOD was inoculated with the non-adapted activated sludge and incubated in the dark. Degradation was followed for 28 days by the continuous automated measurement of BOD. Results showed 4% degradation after 7 and 14 d, 6% after 21 d and 4% after 28 d. The toxicity control determined no toxic effects of the test substance on activated sludge organisms at the tested concentration. Therefore, the test substance is considered to be not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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