Registration Dossier
Registration Dossier
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EC number: 457-830-5 | CAS number: 2557-13-3
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian cell study: DNA damage and/or repair
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- other: Body responsible for the test
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD No. 474 EC 2000/32, B.12 EPA/OPPTS 870.5395
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- -
- EC Number:
- 457-830-5
- EC Name:
- -
- Cas Number:
- 2557-13-3
- Molecular formula:
- Hill formula: C9 H7 F3 O2
- IUPAC Name:
- Methyl, 3-Trifluoromethylbenzoate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- No. of animals per sex per dose:
- Male: 125 mg/kg; No. of animals: 5; Sacrifice time: 24 hours
Male: 250 mg/kg; No. of animals: 5; Sacrifice time: 24 hours
Male: 500 mg/kg; No. of animals: 5; Sacrifice time: 24 hours
Examinations
- Tissues and cell types examined:
- bone marrow of the two femora
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- positive
- Additional information on results:
- Dose producing toxicity in the pre-test for dose selection: 750 mg/Kg body weight
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The single oral administration of Methyl 3-(trifluoromethyl) benzoate did not lead to any increase in the number of polychromatic
erythrocytes containing either small or large micronuclei.The rate of micronuclei was always close to the same range as that of the concurrent negative control in all dose groups and at all sacrifice intervals and within the range of the historical control data.
No relevant inhibition of erythropoiesis determined from the ratio of polychromatic to normochromatic erythrocytes was detected.
Thus, under the experimental conditions chosen here, the test substance does not have any chromosome-damaging (clastogenic) effect,
and there were no indications of any impairment of chromosome distribution in the course of mitosis (aneugenic activity) in bone marrow cells in vivo.
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