Benzoic acid, 3-(trifluoromethyl)-, methyl ester

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC B.13 OECD 471

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

457-830-5

EC Name:

-

Cas Number:

2557-13-3

Molecular formula:

Hill formula: C9 H7 F3 O2

IUPAC Name:

Methyl, 3-Trifluoromethylbenzoate

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

Concentration range in the main test (with metabolic activation): 20 ... 5000 µg/plate
Concentration range in the main test (without metabolic activation): 20 ... 5000 µg/plate

Vehicle / solvent:

Solvent: DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene with S9 mix; N-methyl-N'-nitro-N-nitrosoguanidine, 4-nitro-o-phenylendiamine, 9-aminoacridine and 4-nitroquinoline-N-oxide without S9 mix.

Details on test system and experimental conditions:

Concentration of the test substance resulting in precipitation: 0 µg/plate

Metabolic activation system: The S-9 mix is prepared freshly prior to each experiment
(1,2). For this purpose, a sufficient amount


of S-9 fraction is thawed at room temperature and 1 volume
of S-9 fraction is mixed with 9 volumes of

S-9 supplement (cofactors). This preparation, the so-called
S-9 mix, is kept on ice until used. The

concentrations of the cofactors in the S-9 mix are:

MgCl2 8 mM

KCl 33 mM

Glucose-6-phosphate 5 mM

NADP 4 mM

Phosphate buffer (pH 7.4) 15 mM

The phosphate buffer (6) is prepared by mixing an Na2HPO4
solution with an NaH2PO4 solution in a ratio of about 4 : 1.

To demonstrate the efficacy of the S-9 mix in this assay,
the S-9 batch was characterized with benzo(a)pyrene

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(>=1000 µg/plate)

Test results 2

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(>=1000 µg/plate)

Test results 3

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(>= 1000 µg/plate)

Test results 4

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(>= 1000 µg/plate)

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

The substance did not show any evidence of mutagenic activity in the Salmonella typhimurium/Escherichia coli bacterial system.