Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-[(2-cyanoethyl)[2-(2-hydroxyethoxy)ethyl]amino]-1,3,5-triazine-4,2-diyl]imino]]bis[benzene-1,4-disulphonate]

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From February the 20th to June the 20th, 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -I: Aqueous Exposure Bioconcentration Fish Test

Principles of method if other than guideline:

METI method

GLP compliance:

not specified

Details on sampling:

PREPARATION OF FISH SAMPLE
Fish body weight and length were measured. Tissues were suspended in n-hexane (180 ml), homogenized for 10 minutes and centrifugated (8000 rpm for 5 minutes); at the end of this phase, n-hexane was removed.
Residues were suspended in acetone-chloroform (1 : 1, 180 ml), homogenized for 10 minutes and centrifugated (8000 rpm for 5 minutes); at the end of this phase, acetone-chloroform part was removed.
Residues were suspended in DMF (150 ml), homogenized for 10 minutes and centrifugated (8000 rpm for 10 minutes); the treatment was repeated twice. At the end of this phase, residues were separated.
The test sample was dissolved in DMF, making a volume, analysed by HPLC. After addition of test sample to fish body, the obtained recovery rate was 71.9 % by the above treatment.

PREPARATION OF WATER SAMPLE
10 ml of sample was collected from concentration level 1; condensation was perfromed with 0.5 ml rotary evapourator; after filtration by glass filter, sample was analysed by HPLC.
100 ml of sample was collected from concentration level 2; condensation was perfromed with 0.5 ml rotary evapourator; after filtration by glass filter, sample was cleaned up by coloumn chromatography.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese Killifish.
- Fish weight: 25 g

Route of exposure:

aqueous

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Test temperature:

25.0 ± 0.5 °C

pH:

7.5 ± 3.5

Dissolved oxygen:

6.35 ± 0.85 mg/l

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY
48 hrs TLm > 1000 ppm
During the TL,m test no death occurred at the concentrations of 100, 500 and 1000 ppm.
- Other justification for choice of test concentrations:

Nominal and measured concentrations:

2.0 (concentration level 1) and 0.2 ppm (concentration level 2)

Remarks on result:

other: not indicated

Details on results:

Recovery rate: 71.9 %
The accumulation rate resulted to be lower than ca 0.14 at the concentration level of 2 ppm (corresponding to the actual conc. of 1.7 ppm) and lower than 1.3 at the concentration level of 0.2 ppm.

Test results

Days	Accumulation rate at 2.0 ppm		Accumulation rate at 0.2 ppm
3	A	< 0.14	A'	< 1.3
	B	< 0.15	B'	< 1.2
7	A	< 0.12	A'	< 1.4
	B	< 0.14	B'	< 1.3
14	A	< 0.13	A'	< 1.3
	B	< 0.14	B'	< 1.4
21	A	< 0.15	A'	< 1.3
	B	< 0.12	B'	< 1.2
28	A	< 0.13	A'	< 1.4
	B	< 0.14	B'	< 1.2
42	A	< 0.12	A'	< 1.2
	B	< 0.14	B'	< 1.3
56	A	< 0.13	A'	< 1.3
	B	< 0.16	B'	< 1.4

Actual measured concentration level

Conc. level	1 w	2 w	3 w	4 w	6 w	8 w
At 2.0 ppm	1.8	1.8	1.8	1.8	1.8	1.7
At 0.2 ppm	0.19	0.18	0.18	0.18	0.18	0.18

Conclusions:

The accumulation rate resulted to be lower than ca 0.14 at the concentration level of 2 ppm (corresponding to the actual conc. of 1.7 ppm) and lower than 1.3 at the concentration level of 0.2 ppm.

Executive summary:

The bioaccumulative potential of test item was assessed in Japanese Killifish. The test item concentration was measured in water and in fish tissues, by HPLC method. Based on the TLm value determined in preliminary test, two concentration levels were chosen: 2.0 (concentration level 1) and 0.2 ppm (concentration level 2).

The accumulation rate resulted to be lower than ca 0.14 at the concentration level of 2 ppm (corresponding to the actual conc. of 1.7 ppm) and lower than 1.3 at the concentration level of 0.2 ppm.

Description of key information

No bioaccumulation potential expected.

Additional information

The substance is expected to possess a low potential for bioaccumulation, based on the screening evaluation taken into account the experimental log Pow; on the basis of the molecular mass (i.e. greater than 700) and the steric hindrance, the possible passage of gill membranes or cell membranes of respiratory organs is expected to be negligible.

In addition, the bioaccumulative potential of test item was assessed in Japanese Killifish. The test item concentration was measured in water and in fish tissues, by HPLC method. The accumulation rate resulted to be lower than ca 0.14 at the concentration level of 2 ppm (corresponding to the actual conc. of 1.7 ppm) and lower than 1.3 at the concentration level of 0.2 ppm.