Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-[(2-cyanoethyl)[2-(2-hydroxyethoxy)ethyl]amino]-1,3,5-triazine-4,2-diyl]imino]]bis[benzene-1,4-disulphonate]

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March from the 14th to the 26th, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

1995

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Water solubility:

1 006.4 g/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

7.71 - 7.78

Details on results:

To verify the accuracy of the analytical method a fresh prepared standard solution (prepared with the test item) was measured in between the samples of the flask method.
The recovery rate of the measured standard solutions was in the range from 96.1 to 102.3 %, which shows a good accuracy of the analytical method.

PRELIMINARY TEST
The preliminary test at room temperature showed, that the water solubility of the test item was between 176 and 1057 g/l.

Flask	1	2	3	4
pH value	7.78	7.71	7.73	6.32
measured value [g/l]	379.5 / 384.8	389.7 / 390.9	434.1 / 436.2	-- / --
aliquot of the flask [ml]	1	1	1	1
total volume after 1^stdilution [ml]	50	50	50	50
aliquot of the 1^stdilution [ml]	1	1	1	1
total volume after 2^nddilution [ml]	50	50	50	50
average water solubility [g/l]	955.3	975.9	1087.9	--
mean value of the water solubility [g/l]	1006.4			--
standard deviation [g/l]	71.35			--
relative standard deviation [%]	7.09			--

Conclusions:

1006.4 g/l at 20 °C and pH 7.71-7-78

Executive summary:

The water solubility was determined in accordance with the method and procedures outlined into the OECD guideline 105. For the test item measurement, a specific analytical HPLC-UV method has been validated.

During the preliminary test at room temperature the water solubility of the test item was resulted to be between 176 and 1057 g/l. For the main test three flasks were prepared with an amount of test item and water, and one flask only with water. After stirring and centrifugation the aqueous phases were measured by means of HPLC. The solution of each flask has been measured twice at 20 °C.

The water solubility at 20 °C and pH 7.71 – 7.78 of the test item was determined to be 1006.4 g/l (RSD = 7.09 %)

To verify the accuracy of the analytical method a fresh prepared standard solution (prepared with the test item) was measured in between the samples of the flask method. The recovery rate of the measured standard solutions was in the range from 96.1 to 102.3 %, which shows a good accuracy of the analytical method.

Conclusion

1006.4 g/l at 20 °C and pH 7.71-7-78

Description of key information

1006.4 g/l at 20 °C and pH 7.71-7-78

Key value for chemical safety assessment

Water solubility:

1 006.4 g/L

at the temperature of:

20 °C

Additional information

OECD 105 - Flask method