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EC number: 203-311-1 | CAS number: 105-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study plan 2012-01-09, Performance of study 2012-01-31, Draft report 2012-02-23, Final report 2012-03-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Strain: CHODAT
Family: Chlorophyceae
Order: Chlorococcales
The culture of Desmodesmus subspicatus was obtained in Oct. 2011 by MBM Science-bridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae were kept as stock culture on solid agar at 8 °C. From an aliquot of this stock culture the pre-culture was prepared. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 26 °C
- pH:
- Control at beginning: 7.1; after 72 h: 10.4;
Treatment at beginning: 7.0; after 72 h: 9.9; - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Biomass Integral, area under the curve (AUC)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic potential of the test substance diethyl carbonate was determined in an algal growth inhibition test. The test was performed as limit test at a concentration of 100 mg/L of diethyl carbonate. At this concentration no inhibition was observed. Therefore, the concentration 100 mg/L was stated as NOEC, and all EC50s (growth rate, biomass, yield) were considered to lie above 100 mg/L.
Reference
Results on cell numbers:
The cell numbers were determined by photometric measurement of optical density. The number of cells was slightly higher in the treatment group after 72 hours compared to the control (1,187,567 +/-78,259 cells/mL, 1,122,324 +/-139,063 cells/mL, respectively). The appearance of the alga at the end of the test was analysed microscopically and for the treatment and control group normal growth was observed.
Results on measured concentrations:
The measured concentration of the test substance diethyl carbonate at the beginning of the experiment was 106.78 mg/L and 92.89 mg/L after 72 h. As the analytical determinations of the test substance showed good correlation between nominal and measured concentrations, the determination of the biological results was based on the nominal concentrations.
Results on growth rate, AUC, and yield:
Nominal concentration [mg/L] | growth rate [1/day] | AUC [Cell concentration/(mL*day)] | Yield [Cell concentration/mL] |
0 | 1.84 +/-0.04 | 877,494 +/-90,090 | 1,117,799 +/-139,063 |
100 | 1.86 +/-0.02 | 901,140 +/-55,811 | 1,183,041 +/-78,259 |
Results on the positive control:
At concentrations from 0.04 to 0.25 mg/L of K2Cr2O7 the algal growth was normal compared to the control (0 mg/L K2Cr2O7). At 0.4 and 0.63 mg/L the cells were smaller and at the highest concentrations (1.0, 1.6 mg/L) the cells did not grow anymore (inhibition of growth rate by 48.62 and 54.02 %, respectively).
Description of key information
Determined NOEC in short-term-toxicity study to algae was ≥ 100 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The author of the short-term-toxicity study (72 h) of the test substance diethyl carbonate to algae (Desmodesmus subspicatus) evaluated a NOEC ≥ 100 mg/L.
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