PIGMENT RED 5021B

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-03-06 to 2001-05-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline study (although the study is according to an older OECD 202 version it is similar to the uptodate/revised OECD 202 (2004) , GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

, Part I, 1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations, sampling method, sample storage conditions before analysis:
Composite samples (about 150 mL in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°C until analysis.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 2 g of the test substance were mixed with 18 g of a water solution containing 0.4% lecithin and then diluted at 1 : 100
- Controls: Blank, water and 3.6 mg lecithin/L
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): lecithin
- Concentration of vehicle in test medium (stock solution and final test solution): 4 %
- Evidence of undissolved material (e.g. precipitate, surface film, etc): at the start of exposure small parts of the test substance were observed at the surface of the test solution at test concentrations of nominal 58 and 100 mg/L. A slight deposit was observed at nominal 10 - 58 mg/l and a strong deposit at nominal 100 mg/l at the end of exposure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus 1820
- Source: CIBA-GEIGY Ltd., testing facility
- Age at study initiation: 0-24 hours
- Method of breeding: Cultures of daphnia in 2.5 L glass vessles at 20°C are feed thrice weekly with green algae and a suspension of TETRAMIN-extracts when the water is partly renewed.
- Feeding during test: no

ACCLIMATION
- Acclimation period: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg CaCO3/L

Test temperature:

20 °C

pH:

8.1 to 8.2

Dissolved oxygen:

102 - 104%

Nominal and measured concentrations:

nominal: 10, 18, 32, 58, 100 mg test substance /L
measured: 0.3, 0.6, 0.8, 3.3, 7.7 mg test substance /L at the end of exposure period

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers filled with 100 ml test medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: one daphnid per 25 ml of test solution

TEST MEDIUM / WATER PARAMETERS
Reconstituted test water: analytical grade salts were dissolved in purified water in accordance to the guideline
The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period the test water was not aerated.

OTHER TEST CONDITIONS
- Photoperiod: 16-hour light to 8 hour darkness
- Light intensity: ca. 1500 Lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: none

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 7.7 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: no immobilisation

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

7.7 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: no immobilisation

Reported statistics and error estimates:

Not performed.