PIGMENT RED 5021B

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-08-12 to 1991-10-21

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

OECD guideline study (although the study is according to an older OECD 203 version it is similar to the uptodate/revised OECD 203 (1992) , GLP. Nevertheless, few deviations from the guidelines were reported in the study report (see below in "materials and methods"- Test guideline").

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

, Paris 1984

Deviations:

yes

Remarks:

Fish mean length is 26 mm (22-31 mm). Test concentrations not maintained within the limits given by the guideline.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations, sampling method and sample storage conditions before analysis: Samples (about 150 mL in duplicate) of each test concentration were drawn from the approximate center of the test vessels. They were taken immediately before the exposure and after 96 hours exposure and kept at - 18°C to - 22°C until analysis.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 6 g test substance and 54 g from an aqueous solution containing 0.4 % lecithine were mixed by means of a marble-mill for about 12 h.
- Controls: Blank: dechlorinated tap water, Vehicle: 3.6 mg lecithine/L
- Concentration of vehicle in test medium: 3.6 mg lecithine/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): After calculated amounts of the stock solution (to produce the desired test concentrations) were given into the water and homogeneously distributed and transfered into the aquaria, small parts of the test substance swimming at the surface of the test vessels and a strong deposit were observed at all test times and concentrations.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation: 26 mm (22-31 mm) based on 10 fish
- Weight at study initiation: 0.15 g (0.09-0.27 g) based on 10 fish
- Food type: None

ACCLIMATION
- Acclimation period: 13 days
- Acclimation conditions: 24 hours, no food 24 hours prior to exposure

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

168 mg CaCO/L

Test temperature:

23°C

pH:

7.8 - 8.3

Dissolved oxygen:

84 - 100 %

Nominal and measured concentrations:

Nominal: 10, 18, 32, 58, 100 mg test item/L
Actual: <0.1, <0.1, 0.4, 0.5, 0.4 mg test item/L (see remark in "any other information on materials and methods")

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters. (36/22/25 cm)
- Aeration: No aeration during exposure
- Type of flow-through (e.g. peristaltic or proportional diluter): not indicated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.10 g/L

TEST MEDIUM / WATER PARAMETERS
dechlorinated tap water

OTHER TEST CONDITIONS
- Photoperiod: fluorescent light, 16 hours daily
- Light intensity: not indicated

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: not indicated
- Test concentrations: range of 10-100 mg test item (nominal)/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 0.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: mortality in blank: 0 %, mortality in vehicle: 0 %

Reported statistics and error estimates:

None