Butyryl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

According to: Smith and Carpenter: J. Ind. Hyg. Tox. 26, 269 (1944). According to H.F. Smyth et al: Am. Ind. Hyg. Ass. J. 23, 95-107 (1967).
The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20°C). 3-6 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 3, 10 and 30 minutes. The documentation of clinical signs was performed over a period of 14 days.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butyryl chloride

EC Number:

205-498-5

EC Name:

Butyryl chloride

Cas Number:

141-75-3

Molecular formula:

C4H7ClO

IUPAC Name:

butanoyl chloride

Details on test material:

- Name of test material (as cited in study report): n-buttersäurechlorid
- Substance number: XXVI 622
- Substance type: liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

3-6 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 3, 10 and 30 minutes.

Analytical verification of test atmosphere concentrations:

yes

Remarks on duration:

3, 10 and 30 minutes

Concentrations:

30 min. exposure: 144.3 mg/L
10 min. exposure: 14.4 mg/L
3 min. exposure: 14.0 mg/L

No. of animals per sex per dose:

30 min. exposure: 12
10 min. exposure: 12
3 min. exposure: 6

Control animals:

no

Details on study design:

The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20°C). 3-6 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 3, 10 and 30 minutes. The documentation of clinical signs was performed over a period of 14 days.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 3.6 - < 5.7 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: LC50 calculated using Haber's Law

Mortality:

3/12 animals died when exposed for 3 min to 14.0 mg/l of the test substance
5/6 animals died when exposed for 10 min to 14.4 mg/l of the test substance
5/6 animals died when exposed for 30 min to 144.3 mg/l of the test substance

The following mortalities were observed:
3 min: 25% lethality
10 min: 83% lethality
30 min: 83% lethality
Using a graphical probability analysis, an LT50 value of 5 - 8 min can be estimated. The theoretical vapour saturation concentration in the chamber can be calculated to be ca. 171 mg/l (MW 106.6 and a vapour pressure at 20°C of 39 mbar). Using Haber's Law an LC50 between 3.6 - 5.7 mg/l can be calculated.

Clinical signs:

other: strong attempts to escape, salivation, lacrimation, nasal discharge, dyspnea, cyanose, cloudy cornea

Body weight:

Male mean weight (3-min exposure): 199 g
Male mean weight (10-min exposure): 205 g
Male mean weight (30-min exposure): 188 g
Female mean weight (3-min exosure): 176 g
Female mean weight (10-min exosure): 177 g
Female mean weight (30-min exosure): 173 g

Gross pathology:

Animals that died during the test: hart: acute dilatation, local acute hyperaemia; lungs: emphysema (mid-section), abundant foamy fluid in trachea and bronchi, spotted bleedings in both lobi.
Animals sacrificed at the end of the test: no abnormalities.

Any other information on results incl. tables

The following mortalities were observed:

3 min: 25% lethality

10 min: 83%

30 min: 83%

Using a graphical probability analysis, an LT50 value of 5 - 8 min can be estimated. The theoretical vapour saturation concentration in the chamber can be calculated to be ca. 171 mg/l (MW 106.6 and a vapour pressure at 20°C of 39 mbar). Using Haber's Law an LC50 between 3.6 - 5.7 mg/l can be calculated.