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EC number: 200-680-0
CAS number: 68-19-9
A writhing test was performed in order to determine the antinociceptive
effects of the test item when administered orally to mice, alone or in
combination, for 7 consecutive days. Female mice were exposed to doses
of 215, 1000 and 5620 mg/kg bw/day of the cyanocobalamin alone or in
combination at a ratio of 1:1:0.0025 for thiamine, pyridoxine, and
cyanocobalamin. Control mice received the vehicle. 2h after the last
administration a writhing reaction was induced by intraperitoneal
injection of 10 ml 1% aqueous acetic acid/kg bw. The number of writhing
reactions was monitored for 20 min after the injection in order to
assess the antinociceptive effects of the test item. Following repeated
oral administration, cyanocobalamin alone exerted a slight
antinociceptive effect at 5620 mg/kg bw/day. At this dose level the
number of writhing reactions was reduced by 18% compared to the control.
Cyanocobalamin at a ratio of 1:1:0.0025 (thiamine, pyridoxine, and
cyanocobalamin) increased the antinociceptive effect even further
reducing the number of writhing reactions by 38 and 50% at 1000 and 5620
mg/kg bw/day. From the results of the present study it can be concluded
that there is a slight dose-related antinociceptive effect for
cyanocobalamin at high dose levels in the writhing reaction and that the
addition of cyanocobalamin to the thiamine and pyridoxine combination
resulted in a potentiating effect of cyanocobalamin antinociceptive
effect. No toxic effects were observed at any dose level tested. The
LOAEL was determined to be ≥ 5620 mg/kg bw/day.
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