Propionyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Propionyl chloride

EC Number:

201-170-0

EC Name:

Propionyl chloride

Cas Number:

79-03-8

Molecular formula:

C3H5ClO

IUPAC Name:

propanoyl chloride

Details on test material:

- Analytical purity: 99.3 %
- Name of test material (as cited in study report): propionsäurechlorid
- Lot/batch No.: 96 /561
- Physical state: colorless liquid
- Lot/batch No.: Vers. 127/V
- Storage condition of test material: at room temperature

Method

Target gene:

his

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

Aroclor-induced rat liver S-9 mix

Test concentrations with justification for top dose:

15.625 - 5000 µg/plate (standard plate test)
15.625 - 250 µg/plate (preincubation test)

Vehicle / solvent:

- Vehicle used: DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: with metabolic activation: 2-aminoanthracene (2-AA), without metabolic activation: N-methyl-N' -nitro-N-nitrosoguanidine (MNNG), 4-nitro-o-phenyleridiamine(NOPD) and 9-aminoacridine (AAC) and N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)

Details on test system and experimental conditions:

METHOD OF APPLICATION: standard plate test (SPT) and preincubation test (PIT)
SPT: incubation at 37°C for 48 hours
PIT: preincubation at 37°C for the duration of 20 minutes; incubation at 37°C for 48-72 hours in the dark

NUMBER OF REPLICATIONS: three plates per dose/control

Evaluation criteria:

The test chemical is considered positive when:
A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.

A test substance is generally considered nonmutagenic when:
The number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from 500 μg/plate onward in the standard plate test and at 125 μg - 250 μg/plate in the preincubation test

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from 500 μg/plate onward in the standard plate test and at 125 μg - 250 μg/plate in the preincubation test

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

ADDITIONAL INFORMATION ON CYTOTOXICITY:
A bacteriotoxic effect (reduced his- or trp- background growth, decrease in the number of his+ or trp+ revertants, reduction in the titer) was observed from about 500 μg/plate onward in the standard plate test or at about 125 μg - 250 μg/plate in the preincubation test.

SOLUBILITY:
No precipitation of the test substance was found.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.