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EC number: 700-323-3
CAS number: 908020-52-0
The 72-h ErC50 value is >100 mg/L and the 72-h NOErC is <100 mg/L in Pseudokirchneriella subcapitata
The effect of EEA-NH4 on the growth of the
freshwater microalga Pseudokirchneriella subcapitata was
determined in a GLP-compliant study according to OECD guideline 201
(Kurume Laboratory, 2006d).
In this static limit test algae (initial
cell concentration of 10E+4 cells/mL) were exposed for 72 hours to 100
mg/L EEA-NH4 or a culture medium control (6 replicates per
concentration). The concentrations of test substance were analysed with
high performance liquid chromatography (HPLC) at the start and end of
exposure and determined at 96.4 and 98.6 percent of nominal
Slight, yet significant differences (p<0.05)
were observed on both areas under the growth curves and growth rate
(7.24 and 1.78% respectively). Based on these findings
both the ErC50 and EbC50 were determined at >100 mg/L, and the NOErC and
NOEbC were determined at <100 mg/L.
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