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EC number: 700-323-3
CAS number: 908020-52-0
The 96-h LC50 value is >100 mg/L in medaka (Oryzias latipes).
The acute toxicity of EEA-NH4 to fish was
determined in a GLP-compliant study according to OECD guideline 203 (Kurume
The study was set up as a semi-static limit
test in which 10 fish (Oryzias latipes; 2 replicates of 5
animals) were exposed for 96 hours to 100 mg/L EEA-NH4 or a dilution
water control (dechlorinated tap water). Fatality and visible
abnormality were observed at 3, 24, 48, 72 and 96 hours after the start
of the test. Renewal of the test solution took place after 48 hours. The
concentrations of test substance were analysed with high performance
liquid chromatography (HPLC) at the start of exposure, before renewal,
after renewal and at the end of exposure. Throughout the entirety of the
test the concentration of test item averaged 102% (of the nominal
No mortality or abnormal response was
observed in fish exposed to 100 mg/L EEA-NH4. Based on these findings,
the LC50 was determined to be >100 mg/L.
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