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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: peer reviewed publication, no guideline followed.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrapyrin
EC Number:
217-682-2
EC Name:
Nitrapyrin
Cas Number:
1929-82-4
Molecular formula:
C6H3Cl4N
IUPAC Name:
2-chloro-6-(trichloromethyl)pyridine

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- All crops were individually subjected to the test item in sandy clay loam (3.20 % organic matter). Additionally, Gossypium hirsutun was subjected in various types soil such as sand (0.14 % organic matter), loam type 1 (0.90 % organic matter), sandy loam (2.10 % organic matter), sandy clay loam (3.20 % organic matter), clay loam (4.20 % organic matter) and loam type 2 (28.8 % organic matter).
- Method of application to soil surface: Test item dissolved in vehicle and subsequently titrated to a neutral pH of 7.5 was poured onto air-dried soil samples
- Chemical name of vehicle : Methylene chloride
- Evaporation of vehicle before use: After apllication onto soil, 2 h time-intervall was allowed for complete evaporation of the vehicle.

Test organisms

open allclose all
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Wayne
Species:
other: Medicago sativa
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Ranger
Species:
Lycopersicon esculentum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Pcarson
Species:
Beta vulgaris
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: HH-1
Species:
other: Gossypium hirsutun
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Acala SJ-1
Species:
Oryza sativa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Caloro
Species:
Triticum aestivum
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Sonora
Species:
Sorghum vulgare
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Waconia orange cane
Species:
Zea mays
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Golden Bantam

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Remarks:
14 days: Oryza sativa, Zea mays, Sorghum vulgare m, Triticum aestivum, Gossypium hirsutun, Glycine max (G. soja); 21 days: Medicago sative, beta vulgaris; 24 days: Lycopersicon esculentum;
Post exposure observation period:
no data

Test conditions

Test temperature:
daytime: 26.7 - 32.2°C, nighttime: 18.8 -21.1°C
pH:
no data
Moisture:
no data
Details on test conditions:
TEST SYSTEM
- Testing facility:
- Test container: 0.946 Liter cans
- No. of seeds per container: Corn, cotton, soybean: 8 seeds per container; sorghum, wheat, rice: 15 seeds per container; sugar beet, tomato, alfalfa: 20 seeds per container.
- No. of replicates per treatment group: 1
Nominal and measured concentrations:
no data

Results and discussion

Effect concentrationsopen allclose all
Species:
Zea mays
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
165 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Zea mays
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
20 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Sorghum vulgare
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
120 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Sorghum vulgare
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
50 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Triticum aestivum
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
70 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Triticum aestivum
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
20 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Oryza sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
37 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Oryza sativa
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
10 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
44 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
20 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Lycopersicon esculentum
Duration:
24 d
Dose descriptor:
EC50
Effect conc.:
35 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Lycopersicon esculentum
Duration:
24 d
Dose descriptor:
NOEC
Effect conc.:
5 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
other: Medicago sativa
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
16 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
other: Medicago sativa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
5 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
31 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
10 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
62 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
10 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: plants were grown in sandy clay loam soil
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
33 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: Sand
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
45 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: Loam type 1
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
60 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: Sandy Loam
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
76 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: sandy clay loam
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
230 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: Clay loam
Species:
other: Gossypium hirsutun
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
> 500 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant weight
Remarks on result:
other: Soil: Loam type 2

Applicant's summary and conclusion

Conclusions:
All species tested except Medicago sativa and Lycopersicon esculentum tolerated soil concentrations of the test item which were estimated to be greater than the highest recommended field application rates.
Executive summary:

Studies were conducted to compare the toxicity of the test item to seedlings of various crop plants. For one crop type (cotton) various types of soil were evaluated for comparative purposes. It was observed that the effect of the test item strongly depends on the organic matter content. The toxicity of the decreased with increasing organic matter contents. It was concluded that all species tested except Medicago sativa and Lycopersicon esculentum tolerated soil concentrations of the test item which were estimated to be greater than the highest recommended field application rates.