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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
secondary source
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil. Report EPS Second Draft – August 2005. Method Development and Applications Section, Environmental Technology Centre, Environment Canada, Ottawa, Ontario.
Principles of method if other than guideline:
Study conducted according to Environment Canada test method.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
28 d
Remarks:
Chronic Screening

Test conditions

Test temperature:
20 ± 2°C
pH (if soil or dung study):
Soil pH ranged from 6.19 to 7.64 for the beginning of all tests and from 3.92 to 7.40 for the end of all tests.
Humidity:
Soil moisture content of all treatments in all tests was independent of DEG concentrations and ranged from 59 to116% of the soil water holding capacity (WHC) for the beginning of all tests and from 44 to 91% of the soil WHC for the end of the invertebrate tests.
Photoperiod and lighting:
16 light : 8 dark; Fluorescent, 400-800 Lux
Details on test conditions:
The artificial soil was formulated in the Stantec laboratory from natural ingredients of silica sand (70%), kaolinite clay (20%), and Sphagnum sp. peat (10%) and it was buffered to a neutral pH range (6.0 to 7.5) with calcium carbonate.
Test unit (chamber): Glass 125-mL mason jar
Amount of soil: 30 g wet wt.
Number of replicate test units per treatment: 3
Number of organisms per test unit: 10
Nominal and measured concentrations:
0, 200, 400, 600, 800, 1600, 3200, 6400, 12800, 25600, 51200 mg/kg
The recovery of DEG from the beginning of the range-finding tests was good and ranged from 75 to 90%. DEG recovery on Day 0 of the northern
wheatgrass test was similar among all but the lowest (200 mg/kg) treatments and ranged from 85 to 102%. The recovery in the lowest treatment was below the detection limit. There was evidence of loss of DEG from soil over time; measurable concentrations of DEG were only detected in the highest concentration (25600 mg/kg) at Days 7 and 21 of the test. In addition, the % DEG recovery in these concentrations also decreased over time with 102, 74 and 63% recovery at Days 0, 7 and 21, respectively.

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
LC50
Effect conc.:
15 689 mg/kg soil dw
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
other: IC50
Effect conc.:
7 508 mg/kg soil dw
Basis for effect:
reproduction
Remarks:
number of progeny
Details on results:
Adult Folsomia candida survival was affected adversely following exposure to DEG in AS with a chronic LC50 of 15,689 mg/kg. Chronic reproduction appeared to follow a concentrationdependent pattern and the IC50 for number of progeny produced was 7508 mg/kg.
Adult survival, no. progeny produced

Applicant's summary and conclusion