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Diss Factsheets
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EC number: 204-419-1 | CAS number: 120-71-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed research study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Micronucleus assay using the peripheral blood as described by Hayashi et al., 1990; CSGMT, 1992)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Five male ICR mice were randomly assigned to each treatment group. p-Cresidine was given po twice at 24-h intervals in 10 ml/kg at 250, 500, 1000, or 2000 mg/kg per dose. The treatment range was based on the ip maximum tolerated dose (MTD, >2000 mg/kg), determined by the preliminary acute study. In the positive control group, MMC at 0.5 mg/kg was injected intraperitoneally once. The micronucleus assay using the peripheral blood was performed as described previously (Hayashi et ah, 1990; CSGMT, 1992).
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 6-methoxy-m-toluidine
- EC Number:
- 204-419-1
- EC Name:
- 6-methoxy-m-toluidine
- Cas Number:
- 120-71-8
- Molecular formula:
- C8H11NO
- IUPAC Name:
- 2-methoxy-5-methylaniline
- Details on test material:
- - Name of test material (as cited in study report): p-Cresidine
- Analytical purity: >99%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Mice were used at 8 weeks of age, after 1 week acclimatization period. They were housed at 25 +- 2°C and 55 +-5% humidity under a 12 h light/dark cycle and were given commercial pellet and tap water ad libitum.
Administration / exposure
- Route of administration:
- other: double oral
- Vehicle:
- p-Cresidine and MMC (positive control) were dissolved in olive oil and phsiological saline respectively, immediately before administration
- Duration of treatment / exposure:
- p-Cresidine was given po twice at 24 h intervals in 10 ml/kg at 250, 500, 1000 or 2000 mg/kg per dose
- Frequency of treatment:
- twice at 24 h intervals
- Post exposure period:
- sampling time 24, 48, and 72 h after the second treatment
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
250
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
500
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
1000
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
2000
Basis:
actual ingested
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Positive control(s):
- Mitomycin C at 0,5 mg/kg was injected intraperitoneally once.
Examinations
- Tissues and cell types examined:
- reticulocytes in the mouse peripheral blood
- Evaluation criteria:
- positive: statistically significant increased incidence of micronucleated reticulocytes
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- Two mice died within 24 h of the second dose of p-cresidine at 2000 mg/kg
- Vehicle controls validity:
- not examined
- Negative controls validity:
- other: nagative control = sample before dosing: valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The test chemical does not induce micronulclei in male ICR mice up to the lethal dose. - Executive summary:
In this report, the clastogenicity of p-cresidine was evaluated in the mouse peripheral blood micronucleus test. The test chemical does not induce micronulclei in male ICR mice up to the lethal dose.
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