Hexylamine

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable. Non standard test, not suitable for REACH evaluation.

Data source

Reference

Reference Type:

publication

Title:

An evaluation of the utility of four in vitro short term tests for prediciting the cytotoxicity of individual compounds derived from tobacco smoke

Author:

Curvall M. et al.

Year:

1984

Bibliographic source:

Cell Biology and Toxicology, Vol.1(1), 173-193

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The purpose of the study was to compare 4 in vitro short-term assays predicting the cytotoxic potency of different substances including hexylamine.
Test systems were the followings:
1) Inhibition of cell growth in Ascites sarcoma BP 8 cells,
2) Inhibition of oxidative metabolism in hamster brown fat cells,
3) Membrane damage of human diploid embryonic lung fibroblasts,
4) Inhibition of the ciliary activity using chicken embryo trachea.

GLP compliance:

no

Type of method:

in vitro

Endpoint addressed:

other: cytotoxicity

Test material

Test material information

Constituent 1

Reference substance name:

Hexylamine

EC Number:

203-851-8

EC Name:

Hexylamine

Cas Number:

111-26-2

Molecular formula:

C6H15N

IUPAC Name:

hexan-1-amine

Results and discussion

Details on results:

The relative toxicity is given in mean activity of the substance which is expressed with the aid of the following 10-point scale:
0= 0-9% activity,
1= 10-19% activity,
2= 20-29% and so on.
For hexylamine, values were as follow:
1) Inhibition of cell growth test: 2
2) Inhibition of oxidative metabolism: 2
3) Membrane damage: 9
4) Inhibition of ciliary activity: 0
The mean activity based on the four tests taken together was: 3.3