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EC number: 437-930-5 | CAS number: 51553-03-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 February 2020 - 31 March 2020
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Version / remarks:
- 23 March 2006
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 437-930-5
- EC Name:
- -
- Cas Number:
- 51553-03-8
- Molecular formula:
- Hill formula: C22H23N7O5 CAS formula: C22H23N7O5
- IUPAC Name:
- N-{5-[bis(2-methoxyethyl)amino]-2-[2-(2,6-dicyano-4-nitrophenyl)diazen-1-yl]phenyl}acetamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the test and at day 2, 5 and at the end of the test (day 7) the frond number and the frond area are determined using the digital image evaluation system Scanalyzer from LemnaTec (Würselen). The test endpoint is inhibition of the average specific growth rates and the yield of frond number and frond area during the exposure period. A static test design was applied with an accompanying substance specific analysis. A nominal loading rate of 100 mg/L test item was used to prepare a saturated solution of the test item. The saturated solution was filtered with a 0,45 μm aPES membrane filter to remove undissolved particles and diluted with test medium to obtain the following concentrations: 6.25 %, 12.5 %, 25 %, 50 % and 100 % (v/v) of the saturated solution. A preliminary range finding test without GLP was performed before the start of this GLP-study. Nominal loading rates of 10 mg/L and 100 mg/L test item were prepared, shaken for 24 h and filtered with a 0,45 μm a PES membrane filter. The filtered saturated solution with nominal loading rates of 10 mg/L and 100 mg/L resulted in 0 % effect.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test was performed with Steinberg medium.
Test organisms
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- Test was carried out with the duckweed Lemna minor obtained from the German Federal Environment Agency (UBA), Berlin-Marienfelde. The sterile permanent stock culture is grown on agar 2 medium prepared according to Jungnickel & Augsten (Details see Appendix I) in 100 mL sealed Erlenmeyer flasks at room temperature. After a maximum period of 2 months the plants are transferred sterile into freshly prepared medium. The stock culture used in the test has been cultivated at Hydrotox since March 2014. 14 days before the start of the test, plant colonies were transferred from the sterile agar stock culture into 150 mL beakers containing 100 mL Steinberg Medium. After 7 days they were transferred into fresh medium and grown until being used for the test. Colonies consisting of 2 to 4 visible fronds were transferred from the inoculum culture and randomly assigned to the test vessels under aseptic conditions. A quality check takes place at regular intervals using a concentration range of 3,5-dichlorophenol (Sigma-Aldrich, Steinheim, Germany, Lot No. MKBS0425V, from September 2015) in order to assess the validity of the test organism. The last quality check was performed in November 2019. The EC50 for the frond number was 2.96 mg/L (2.46 – 3.54 mg/L 95%-CI). This value is in-line with the requirements of the ISO 20079 (2005) (Lemna, Growth Inhibition Test] of 2.2 –3.8 mg/L).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 7 d
Test conditions
- Test temperature:
- 24 - 25 °C
- pH:
- 5,3 - 5,8
- Conductivity:
- 99 μE/m² s
- Nominal and measured concentrations:
- 100 mg/L nominal loading rate to prepare a saturated solution,
tested concentrations: 6.25 %, 12.5 %, 25 %, 50 % and 100 %
(v/v) of the saturated solution - Details on test conditions:
- 100 mL from the test and negative control solutions were dispensed into 150 mL beakers. Test solutions were prepared with 3 replicates, negative control was prepared with 6 replicates. Two additional replicates of each treatment group were prepared without test organism for the chemical analysis. Lemna plant colonies with a total number of 9 – 10 fronds were transferred from the preculture into the test and control vessels at the start of the test. Test vessels were covered with watch glasses to minimize evaporation from the test vessels.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 other: v/v %
- Nominal / measured:
- nominal
- Conc. based on:
- other: saturated solution
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 other: v/v %
- Nominal / measured:
- nominal
- Conc. based on:
- other: saturated solution
- Basis for effect:
- growth rate
- Details on results:
- Since test item concentrations were below the limit of calibration, the results are given in relation to the concentration of the saturated solution that was prepared by shaking a loading rate of 100 mg/L for 24 h and removing undissolved particles by filtration.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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