Carbonic acid, 4-nitrophenyl 5-thiazolylmethyl ester, hydrochloride (1:1)

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 October - 15 November, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 202 Part 2 (Testing of Chemicals)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

414-490-2

EC Name:

-

Cas Number:

154212-59-6

Molecular formula:

C11H9ClN2O5S

IUPAC Name:

4-nitrophenyl (1,3-thiazol-5-yl)methyl carbonate hydrochloride

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations (measured): 0.036, 0.066, 0.125, 0.270, 0.415mg/l plus Control plus Solvent Control.
- Sampling method: Duplicate aqueous samples (40 to 50ml) of solvent control and each treatment solution were analyzed immediately after preparation on days 0, 3, 7, 14, 17 and 19
- Sample storage conditions before analysis: 20±2°C

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain/clone: Straus
- Source: Institut National de Recherche Chimique Applique (IRChA) France
Feeding during test
- Food type: green alga Chlorella vulgaris strain CCAP211/12
- Frequency: 0.5 - 1.0ml at least 5 times weekly

Study design

Test type:

other: treatment solutions renewed 3 times per week.

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

20±2°C

pH:

7.9 - 8.0

Dissolved oxygen:

8.4 - 8.7mgO2/L

Nominal and measured concentrations:

Measured concentrations of test substance were between 4 - 36 % of the nominal values.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: 1 litre glass beakers with loosely fitted foam bung.
- Material, size, headspace, fill volume: 500ml Elendt M4 culture medium
- Aeration:
- Renewal rate of test solution (frequency/flow rate): 3/wk on days 3, 5, 7, 10, 12, 14, 17 and 19.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4


TEST MEDIUM / WATER PARAMETERS
Diluent water control and solvent control treatmentswere prepared. Solvent controls contained 100µl DMF:Tween 80 as auxillary solvent.

OTHER TEST CONDITIONS
- Photoperiod: 16hrs light, 8hrs dark

Reference substance (positive control):

yes

Remarks:

100µl DMF:Tween 80

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.45 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other: parental mortality

Remarks on result:

other: 95%CL: 0.35 -0.57mg/L

Effect concentrations 2

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

0.33 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95%CL:0.30 - 0.37mg/l

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.34 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95%CL: 0.32 - 0.37mg/l

Effect concentrations 4

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.125 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

Values based on mean measured concentrations calculated .
Limit of detection: 0.02mg/l

Reported statistics and error estimates:

Mean measured concentrations of A-87439.1 in test solutions on each sampling occassion were variable and ranged from 54.8% to 126% of nominal for freshly prepared solutions.
Analysis of solvent stock solutions of A-87439.1 showed the mean measured concentration to be 95.3% of nominal.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Prolonged exposure of Daphnia magna to A-87439.1 caused inhibition of reproduction with EC 50 0.34 mg/l and NOEC of 0.125 mg/l measured concentrations. LOEC was 0.270 mg/L.