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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
8 December 2009 - 17 December 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
The study report includes a certificate of GLP compliance issued by the MHRA.
Type of method:
HPLC estimation method
Media:
soil
Radiolabelling:
no
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Agilent 1200 Series Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: Hypersil CPS (25 cm x 4.6 mm internal diameter)
- Column temperature: 25°C
- Flow rate: 1.0 ml/minute
- Injection volume: 20 µl
- Detection system: UV set at 210 nm

MOBILE PHASES
- Type: Acetonitrile:water (55:45 v/v)
- Experiments with additives carried out on separate columns: N/A
- pH: no data
- Solutes for dissolving test and reference substances: acetonitrile:water (3:1 v/v)

DETERMINATION OF DEAD TIME
- Method: An internal deadtime standard (IDS) was used - formamide.

REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20µL of a solution at 1.6g/L
- Quantity of reference substances: 20µL of a solution at 1.6g/L
- Intervals of calibration: no data

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': yes
- Calculation of retention times:yes
- Determination of the log Koc value: yes
Key result
Type:
log Koc
Value:
1.2
Temp.:
25 °C
Details on results (HPLC method):
- Retention times of reference substances used for calibration:
Deadtime standard - 2.90 minutes
Acetanilide - 3.42 minutes
Nitrobenzene - 4.10 minutes
Napthalene - 4.72 minutes
2-Methylnapthalene - 4.98 minutes
Anthracene - 5.50 minutes
DDT - 7.87 minutes
- Details of fitted regression line (log k' vs. log Koc): calculated regression - log10Koc = 4.62log10k + 4.37
- Average retention data for test substance: retention time for substance = 3.501 minutes; calculated log10Koc = 1.2
Validity criteria fulfilled:
yes
Conclusions:
MMB-Ac was found to have log Koc value of 1.2. at 25°C.

Description of key information

MMB-Ac was found to have log Koc value of 1.2. at 25°C, which equalizes to a Koc value of 15.8 L/kg.

Key value for chemical safety assessment

Other adsorption coefficients

Type:
log Kp (solids-water in soil)
Value in L/kg:
1.2
at the temperature of:
25 °C

Additional information