Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 213-485-0 | CAS number: 957-68-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 26, 2012 to October 24, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study (DIN 9408) with acceptable restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9408 (Ultimate Aerobic Biodegradability - Method by Determining the Oxygen Demand in a Closed Respirometer)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- EC Number:
- 213-485-0
- EC Name:
- 3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- Cas Number:
- 957-68-6
- Molecular formula:
- C10H12N2O5S
- IUPAC Name:
- (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name: 7-Aminocephalosporansäure
Lot: B146988
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial, adapted
- Details on inoculum:
- Source and type: Cleaned activated adapted sludge from the industrial sewage plant ARA of the Industriepark Höchst
Used amount: 13.7 mL/L sludge suspension
Content of solid: 2.2 g/L (dry substance)
Content in the end testing volume: 30 g/L (dry substance)
Acclimatization status: Not acclimatized to the test item and reference substance - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- other: calculated from TOC and Purity of the test item
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Testing temperature: 22.9 - 23.4°C
pH value: 7.3 - 7.6
Preparation volume: 0.365 L in 1L-Bottles with magnetic stirrers, connected to the respirometer unit
Analytics of the Degradability: Measurement of the biochemical O2-requirement by measuring the pressure differences in aclosed respirometer cell
Analytics of the elimination: Determination of the CSB in O2/L
Control preparations:
reference substance: 100mg/L in dilution water
abiotic control: test preparation without inokulum, with addition of 2x100 mg/L HgCl2 during test performance
Inhibition control: 100 mg/L reference substance and 100 mg/L test item in dilution water
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/l in dilution water
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 30
- Sampling time:
- 28 d
- Details on results:
- Due to the elimination caused by the abiotic control (elimination 21% after 3 days, 28% after 28 days) no evaluation of the biological degradability of the test item can be stated.
For the results of the elimination the test item can be evaluated as moderately eliminable under the test conditions.
The elimination rate DThSB was 30% after 28 days, the CSB-elimination came up to 31% after 28 days.
BOD5 / COD results
- Results with reference substance:
- The degradability of the reference substance was >60% after 14 days.
The rate of degradability of the inhibition control was >40%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: 7-ACA is moderately eliminable under the test conditions
- Conclusions:
- Due to the elimination caused by the abiotic control (elimination 21% after 3 days, 28% after 28 days) no evaluation of the biological degradability of the test item can be stated.
For the results of the elimination the test item can be evaluated as moderately eliminable under the test conditions.
The elimination rate DThSB was 30% after 28 days, the CSB-elimination came up to 31% after 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
