3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Density

Administrative data

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 01, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study (OECD109) with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7300 (Density / Relative Density / Bulk Density)

Deviations:

no

GLP compliance:

no

Type of method:

air comparison pycnometer (for solids)

Test material

Test material information

Constituent 1

Reference substance name:

3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

EC Number:

213-485-0

EC Name:

3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Cas Number:

957-68-6

Molecular formula:

C10H12N2O5S

IUPAC Name:

(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Name: 7-Aminocefalosporansäure (7-ACA)
Lot no.: B146988

Results and discussion

Density

Type:

relative density

Density:

1.51

Temp.:

20 °C

Any other information on results incl. tables

Two tests with different amounts of the test item were performed to determine the relative density D₄²⁰at a temperature of 20 °C ±0.01 °C.Table1summarises the results.

 

Table1:      Results of the measurement of the density of the test item

Test No.	Temperature [°C]	Test item chamber volume	Reference chamber volume	Weight in of test item [g]	Density [g/cm³]*
1	20	Small (25.62 cm³)	76 (21.35 cm³)	11.34	1.50933
2	20	Small (25.62 cm³)	76 (21.35 cm³)	10.96	1.50595

* The pressurizing-expansion cycles are performed until 5 subsequent volume measurements have a relative standard deviation (RSD) of ≤ 0.02 %.

 

The mean density of the test item at 20 °C is 1.507677692 g/cm³ (SD = 0.0024, RSD = 0.16 %). The relative density D₄²⁰is calculated by dividing the density of the test item by the density of water at 4 °C (0.9999750 g/cm³).

 

The relative density is                             D₄²⁰= 1.51.

Applicant's summary and conclusion

Conclusions:

According to OECD Test Guidelines 109 (1995) and EC 440/2008 Part A. Method A.3., the relative density D420 of the test item 7-Aminocephalosporanic acid at 20 °C ±0.01 °C (compared to water at 4 °C) is D420 = 1.51.

Executive summary:

The density of solids is determined with a calibrated gas comparison pycnometer at a temperature of 20 °C ±0.01 °C. A pressurized gas (He) is expanded from a pressurized reference chamber into a sample chamber in which the test item is placed under atmospheric pressure. The volume of the sample chamber (25.62 cm³) as well as the volume of the reference chamber (21.35 cm³) is exactly known. The free volume of the sample chamber in which the gas is expanded can be calculated by the ideal gas law. With the free gas volume of the sample chamber, the volume of the test item in the sample chamber can be calculated by subtracting the free gas volume from the total chamber volume. By measuring the mass of the test item, the density is determined. The relative density D₄²⁰is obtained by dividing the measured density of the test-item by the density of water at 4 °C (0.9999750 g/cm³)