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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
09 February 1981 (started); 01 April 1981 (reported)
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
other: research report
Title:
Unnamed
Report date:
1987

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
no
Remarks:
study was conducted before implementation of GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[[3-(dimethylamino)propyl]methylamino]ethanol
EC Number:
279-903-9
EC Name:
2-[[3-(dimethylamino)propyl]methylamino]ethanol
Cas Number:
82136-26-3
Molecular formula:
C8H20N2O
IUPAC Name:
2-{[3-(dimethylamino)propyl](methyl)amino}ethan-1-ol
Details on test material:
- Name of test compound: ABBOTT-51806
- Lot No. 7753-37

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
Skin of back was shaved and, following application of the test material, was wrapped with occlusive film for 24 hours.
Duration of exposure:
24 hours
Doses:
1.59 mL/kg bw, 2.52 mL/kg bw and 4.00 mL/kg bw [dose volume was calculated as dose level (g/kg) / density (g/mL)]
No. of animals per sex per dose:
6
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
1.96 mL/kg bw
Based on:
test mat.
95% CL:
0.81 - 2.73
Remarks on result:
other: abraded skin
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
1 772 mg/kg bw
Based on:
test mat.
Remarks on result:
other: abraded skin
Sex:
male
Dose descriptor:
LD50
Effect level:
2 mL/kg bw
Based on:
test mat.
95% CL:
1.4 - 2.72
Remarks on result:
other: intact skin
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
1 808 mg/kg bw
Based on:
test mat.
Remarks on result:
other: intact skin
Mortality:
1.59 mL/kg bw: 1/6
2.52 mL/kg bw: 5/6
4.00 mL/kg bw: 6/6

Any other information on results incl. tables

Signs of toxicity were usually not observed until the day after the material was applied. Local effects were not reversible within the two-week observation period. Deaths occurred 1 to 4 days post treatment.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
- LD50 (male, abraded skin): 1772 mg/kg bw;
- LD50 (male, intact skin): 1808 mg/kg bw
Executive summary:

The test item 2-[[3-(dimethylamino)propyl]methylamino]ethanol was tested in an acute dermal toxicity study [equivalent or similar to OECD Guideline 402 (before 9 Oct. 2017), prior to introduction of GLP].


The test material was administered to six male New Zealand White rabbits by a single dermal application with 1.59 mL, 2.52 mL and 4.00 mL of the undiluted test material per kg body weight for 24 hours.


Signs of toxicity were usually not observed until the day after the material was applied. Local effects were not reversible within the two-week observation period. Deaths occurred 1 to 4 days post treatment.


The acute dermal median lethal dose (LD50) of the test material in the rabbit was estimated to be 1772 mg/kg bw (abraded skin) and 1808 mg/kg bw (intact skin).


Using the EU Globally Harmonised System (GHS), the substance needs to be classified as Acute tox. 4, H312.